Study of the binding of Eu3+ and Tb3+ to L-phenylalanine and L-tryptophan

被引:16
作者
Carubelli, CR [1 ]
Massabni, AMG [1 ]
Leite, SRDA [1 ]
机构
[1] Univ Estadual Paulista, Inst Quim, BR-14801970 Araraquara, SP, Brazil
关键词
aromatic amino acids; lanthanides;
D O I
10.1590/S0103-50531997000600006
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Trivalent europium and terbium ions have ionic radii similar to that of Ca2+. So they are employed as probes of calcium binding sites in biological molecules. These ions exhibit very useful spectroscopic characteristics, chiefly a pronounced luminescence. In protein bound lanthanide, visible light emission from the lanthanide excited states can be observed when UV light is absorbed by aromatic amino acids. Subsequently, the energy is transferred to the lanthanide ion. The present work was carried out to define the binding sites of Eu3+ and Tb3+ in complexes with the aromatic amino acids L-phenylalanine and L-tryptophan. The techniques utilized were infrared and C-13 nuclear magnetic resonance spectroscopies. It was found that trivalent europium and terbium interact with the carboxylate group of both amino acids. With L-tryptophan, the imino group of the indole ring is also involved representing another coordination site.
引用
收藏
页码:597 / 602
页数:6
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