Next Generation Sequencing of Actinobacteria for the Discovery of Novel Natural Products

被引：85

作者：

Gomez-Escribano, Juan Pablo ^{[1
]}

Alt, Silke ^{[1
]}

Bibb, Mervyn J. ^{[1
]}

机构：

[1] John Innes Ctr Plant Sci Res, Dept Mol Microbiol, Norwich Res Pk, Norwich NR4 7UH, Norfolk, England

来源：

MARINE DRUGS | 2016年 / 14卷 / 04期

基金：

英国生物技术与生命科学研究理事会;

关键词：

actinomycetes; Streptomyces; genome mining; PacBio; Illumina; next generation sequencing; BIOSYNTHETIC GENE CLUSTERS; COMPLETE GENOME SEQUENCE; STREPTOMYCES-COELICOLOR; HETEROLOGOUS EXPRESSION; ERROR-CORRECTION; CODON USAGE; IDENTIFICATION; REVEALS; EXPLOITATION; SALINISPORA;

D O I：

10.3390/md14040078

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Like many fields of the biosciences, actinomycete natural products research has been revolutionised by next-generation DNA sequencing (NGS). Hundreds of new genome sequences from actinobacteria are made public every year, many of them as a result of projects aimed at identifying new natural products and their biosynthetic pathways through genome mining. Advances in these technologies in the last five years have meant not only a reduction in the cost of whole genome sequencing, but also a substantial increase in the quality of the data, having moved from obtaining a draft genome sequence comprised of several hundred short contigs, sometimes of doubtful reliability, to the possibility of obtaining an almost complete and accurate chromosome sequence in a single contig, allowing a detailed study of gene clusters and the design of strategies for refactoring and full gene cluster synthesis. The impact that these technologies are having in the discovery and study of natural products from actinobacteria, including those from the marine environment, is only starting to be realised. In this review we provide a historical perspective of the field, analyse the strengths and limitations of the most relevant technologies, and share the insights acquired during our genome mining projects.

引用

页数：19

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