Lack association of eNOS (G894T) and p22phox NADPH oxidase subunit (C242T) polymorphisms with systemic sclerosis in a cohort of French Caucasian patients

被引：16
作者：
Allanore, Y
Borderie, D
Lemaréchal, H
Ekindjian, OG
Kahan, A
机构：
[1] Univ Paris 05, Dept Rheumatol A, Assistance Publ Hop Paris, Hop Cochin, F-75679 Paris 14, France
[2] Hop Cochin, Assistance Publ Hop Paris, Dept Biochem A, F-75679 Paris 14, France
来源：
CLINICA CHIMICA ACTA | 2004年 / 350卷 / 1-2期
关键词：
systemic sclerosis; nitric oxide synthase; NADPH oxidase; polymorphisms;
D O I：
10.1016/j.cccn.2004.07.008
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Objective: To assess the influence of endothelial nitric oxide synthase (eNOS) and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase polymorphisms on the susceptibility of patients to and clinical expression of systemic sclerosis (SSc). Methods: Seventy-seven French Caucasian patients with SSc were studied. Patients and ethnically matched controls (n = 49) were genotyped, by restriction enzyme digestion of polymerase chain reaction (PCR) products, for G894T polymorphism in exon 7 of the eNOS gene and for C242T polymorphism of the gene encoding the p22(phox) NADPH oxidase subunit. Results: The allele and genotype frequencies of the polymorphisms did not differ between patients with SSc and the controls. Moreover, there was no association between these polymorphisms and disease phenotypes. Conclusion: Our results indicate that eNOS (G894T) and p22(phox) (C242T) polymorphisms do not influence susceptibility to and the course of systemic sclerosis. (C) 2004 Elsevier B.V. All rights reserved.
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页码：51 / 55
页数：5
相关论文
共 15 条
[1]   Acute and sustained effects of dihydropyridine-type calcium channel antagonists on oxidative stress in systemic sclerosis [J].
Allanore, Y ;
Borderie, D ;
Lemaréchal, H ;
Ekindijan, OG ;
Kahan, A .
AMERICAN JOURNAL OF MEDICINE, 2004, 116 (09) :595-600
[2]   PREDICTORS OF SURVIVAL IN SYSTEMIC-SCLEROSIS (SCLERODERMA) [J].
ALTMAN, RD ;
MEDSGER, TA ;
BLOCH, DA ;
MICHEL, BA .
ARTHRITIS AND RHEUMATISM, 1991, 34 (04) :403-413
[3]   Genotyping method for point mutation detection in the endothelial nitric oxide synthase exon 7 using fluorescent probes. Clinical validation in systemic sclerosis patients [J].
Biondi, ML ;
Marasini, B ;
Leviti, S ;
Turri, O ;
Bernini, M ;
Seminati, R ;
Porreca, W ;
Guagnellini, E .
CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 2001, 39 (03) :281-282
[4]   A variant of p22phox, involved in generation of reactive oxygen species in the vessel wall, is associated with progression of coronary atherosclerosis [J].
Cahilly, C ;
Ballantyne, CM ;
Lim, DS ;
Gotto, A ;
Marian, AJ .
CIRCULATION RESEARCH, 2000, 86 (04) :391-395
[5]  
Cerinic MM, 2002, RHEUMATOLOGY, V41, P843
[6]   The Glu298Asp polymorphism in the endothelial nitric oxide synthase gene is strongly associated with coronary spasm [J].
Chang, KY ;
Baek, SH ;
Seung, KB ;
Kim, PJ ;
Ihm, SH ;
Chae, JS ;
Kim, JH ;
Hong, SJ ;
Choi, KB .
CORONARY ARTERY DISEASE, 2003, 14 (04) :293-299
[7]   High prevalence of polymorphisms of angiotensin-converting enzyme (I/D) and endothelial nitric oxide synthase (Glu298Asp) in patients with systemic sclerosis [J].
Fatini, C ;
Gensini, F ;
Sticchi, E ;
Battaglini, B ;
Angotti, C ;
Conforti, ML ;
Generini, S ;
Pignone, A ;
Abbate, R ;
Matucci-Cerinic, M .
AMERICAN JOURNAL OF MEDICINE, 2002, 112 (07) :540-544
[8]   Emerging potentials for an antioxidant therapy as a new approach to the treatment of systemic sclerosis [J].
Gabriele, S ;
Alberto, P ;
Sergio, G ;
Fernanda, F ;
Marco, MC .
TOXICOLOGY, 2000, 155 (1-3) :1-15
[9]  
Guzik TJ, 2000, CIRCULATION, V102, P1744
[10]  
Harrison David G., 2003, Cardiology Clinics, V21, P289, DOI 10.1016/S0733-8651(03)00073-0
12