Preparation of low-molecular-weight polyguluronate sulfate and its anticoagulant and anti-inflammatory activities

被引：58
|
作者：
Zhao, Xia [1 ]
Yu, Guangli
Guan, Huashi
Yue, Nan
Zhang, Zhenqing
Li, Haihua
机构：
[1] Ocean Univ China, Inst Marine Drug & Food, Qingdao 266003, Peoples R China
[2] Tianjin Inst Pharmaceut Res, Tianjin 300193, Peoples R China
来源：
CARBOHYDRATE POLYMERS | 2007年 / 69卷 / 02期
关键词：
polyguluronate sulfate; alginate; anticoagulant activity; anti-inflammatory activity;
D O I：
10.1016/j.carbpol.2006.10.024
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Low-molecular-weight polyguluronate sulfate (LPGS) was prepared as a heparinoid by chemical sulfation of polyguluronic acid (PG), which was isolated from the hydrolysate of alginate by pl-l-fractionated precipitation. The structure of LPGS was elucidated based on FT-IR and 2D NMR spectroscopy. Sulfation was demonstrated to occur at the C-2 and C-3 positions of the guluronic acid residues and the degree of substitution (DS) was 1.53 per monosaccharide residue. The weight-averaged molecular weight of LPGS was 11.4 kDa determined by high performance gel permeation chromatography (HPGPC). The in vitro anticoagulant activity and in vivo anti-inflammatory activity of LPGS were evaluated. Results showed that LPGS significantly prolonged the whole blood clotting time and activated partial thrombinplastin time (APTT), and exhibited considerable anti-inflammatory activity in cotton pelletinduced granuloma in rats. (C) 2006 Elsevier Ltd. All rights reserved.
引用
收藏
页码：272 / 279
页数：8
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