Comparative analysis of Bombyx mori nucleopolyhedrovirus responsive genes in fat body and haemocyte of B-mori resistant and susceptible strains

被引：59
作者：
Bao, Y-Y. [1 ]
Lv, Z-Y. [1 ]
Liu, Z-B. [1 ]
Xue, J. [1 ]
Xu, Y-P. [1 ]
Zhang, C-X. [1 ]
机构：
[1] Zhejiang Univ, Minist Agr, Key Lab Mol Biol Crop Pathogens & Insects, Inst Insect Sci, Hangzhou 310029, Zhejiang, Peoples R China
来源：
INSECT MOLECULAR BIOLOGY | 2010年 / 19卷 / 03期
基金：
中国国家自然科学基金;
关键词：
BmNPV; Bombyx mori; fat body; haemocyte; immune defence response; HEAT-SHOCK PROTEINS; TRANSCRIPT LEVELS; INFECTION; HOST; EXPRESSION; GLOVERIN; SILKWORM; VIRUS;
D O I：
10.1111/j.1365-2583.2010.00993.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The infection profiles of the Bombyx mori nucleopolyhedrovirus (BmNPV) in B. mori larvae revealed that the virus invaded the fat body and haemocyte of both KN and 306 strains, which are highly resistant and susceptible, respectively, to BmNPV infection. However, viral proliferation was notably slowed in the resistant B. mori strain. Using suppression subtractive hybridization, two fat body cDNA libraries were constructed to compare BmNPV responsive gene expression levels between the two silkworm lines. In total, 96 differentially expressed genes were obtained. Real-time quantitative PCR (qPCR) analysis confirmed that eight genes were significantly up-regulated in the fat body and haemocyte of the KN strain following BmNPV injection. Our results suggest that these genes may have potential roles in B. mori antiviral infection mechanisms.
引用
收藏
页码：347 / 358
页数：12
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