Geranylgeraniol enhances testosterone production via the cAMP/protein kinase A pathway in testis-derived I-10 tumor cells

被引：27

作者：

Ho, Hsin-Jung ^{[1
]}

Shirakawa, Hitoshi ^{[1
]}

Yoshida, Risa ^{[1
]}

Ito, Asagi ^{[1
]}

Maeda, Misato ^{[1
]}

Goto, Tomoko ^{[1
]}

Komai, Michio ^{[1
]}

机构：

[1] Tohoku Univ, Grad Sch Agr Sci, Lab Nutr, Sendai, Miyagi 980, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2016年 / 80卷 / 04期

基金：

日本学术振兴会;

关键词：

geranylgeraniol; cAMP; protein kinase A; testosterone; progesterone; FACTOR-KAPPA-B; JAPANESE MEN; SUPPRESSES; EXPRESSION; LEVEL; INHIBITION; ACTIVATION;

D O I：

10.1080/09168451.2015.1123612

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Testosterone levels in men decrease with age; this decline has been linked to various diseases and can shorten life expectancy. Geranylgeraniol (GGOH) is an isoprenoid found in plants that plays an important role in several biological processes; however, its role in steroidogenesis is unknown. Here, we report that GGOH enhances the production of testosterone and its precursor progesterone in testis-derived I-10 tumor cells. GGOH induced protein kinase A (PKA) activity and increased cAMP levels and was found to regulate cAMP/PKA signaling by activating adenylate cyclase without altering phosphodiesterase activity. GGOH also stimulated mRNA and protein levels of steroidogenic acute regulatory protein, a downstream effector in the cAMP/PKA pathway. These results demonstrate that GGOH enhances steroidogenesis in testis-derived cells by modulating cAMP/PKA signaling. Our findings have potential applications for the development of therapeutics that increase testosterone levels in aging men.

引用

页码：791 / 797

页数：7

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