Growth factor production from fibrin-encapsulated human keratinocytes

被引：7

作者：

Acevedo, Cristian A. ^{[1
]}

Somoza, Rodrigo A. ^{[1
]}

Weinstein-Oppenheimer, Caroline ^{[2
]}

Brown, Donald I. ^{[3
]}

Young, Manuel E. ^{[1
]}

机构：

[1] Univ Tecn Federico Santa Maria, Ctr Biotecnol, Valparaiso, Chile

[2] Univ Valparaiso, Fac Farm, Dept Bioquim, Valparaiso, Chile

[3] Univ Valparaiso, Fac Ciencias, Dept Biol & Ciencias Ambientales, Valparaiso, Chile

来源：

BIOTECHNOLOGY LETTERS | 2010年 / 32卷 / 07期

关键词：

Fibrin; Growth factors; Keratinocytes; HUMAN DERMAL FIBROBLASTS; CELL; GLUE; MICE;

D O I：

10.1007/s10529-010-0245-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Fibrin has been used extensively in cell encapsulation because it has important biological properties. Keratinocyte encapsulation in fibrin is a widely used technique in skin tissue engineering. The production of growth factors (EGF, TGF-beta 1 and PDGF-BB) was evaluated when keratinocytes are encapsulated in fibrin. Secretions of TGF-beta 1 and PDGF-BB increased more than five times compared to monolayer cultures. Encapsulated cells secreted about 80% active form of TGF-beta 1 (monolayer cells only secreted inactive form). An enhanced secretion of TGF-beta 1 and PDGF-BB was found in encapsulated cells, showing that fibrin capsules are favourable for the production of these growth factors.

引用

页码：1011 / 1017

页数：7

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