Immobilization of invertase in conducting copolymers of 3-methylthienyl methacrylate

被引：34
作者：
Cirpan, A
Alkan, S
Toppare, L [1 ]
Hepuzer, Y
Yagci, Y
机构：
[1] Middle E Tech Univ, Dept Chem, TR-06531 Ankara, Turkey
[2] Istanbul Tech Univ, Dept Chem, TR-80626 Istanbul, Turkey
来源：
BIOELECTROCHEMISTRY | 2003年 / 59卷 / 1-2期
关键词：
immobilization; invertase; conducting copolymer; electropolymerization; polythiophene; polypyrrole;
D O I：
10.1016/S1567-5394(02)00186-X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Immobilization of invertase in conducting copolymer matrices of 3-methylthienyl methacrylate with pyrrole and thiophene was achieved by constant potential electrolysis using sodium dodecyl sulfate (SDS) as the supporting electrolyte. Polythiophene (PTh) was also used in entrapment process for comparison. Kinetic parameters, Michaelis-Menten constant, K-m, and the maximum reaction rate, V-max, were investigated. Operational stability and temperature optimization of the enzyme electrodes were also examined. (C) 2002 Elsevier Science B.V. All rights reserved.
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收藏
页码：29 / 33
页数：5
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