The Seed Development Factors TT2 and MYB5 Regulate Heat Stress Response in Arabidopsis

被引：29

作者：

Jacob, Pierre ^{[1
]}

Brisou, Gwilherm ^{[1
]}

Dalmais, Marion ^{[1
]}

Thevenin, Johanne ^{[2
]}

van der Wal, Froukje ^{[3
,4
]}

Latrasse, David ^{[1
]}

Devani, Ravi Suresh ^{[1
]}

Benhamed, Moussa ^{[1
]}

Dubreucq, Bertrand ^{[2
]}

Boualem, Adnane ^{[1
]}

Lepiniec, Loic ^{[2
]}

Immink, Richard G. H. ^{[3
,4
]}

Hirt, Heribert ^{[5
,6
]}

Bendahmane, Abdelhafid ^{[1
]}

机构：

[1] Univ Paris Saclay, Univ Evry, Inst Plant Sci Paris Saclay, INRAE,CNRS, F-91405 Orsay, France

[2] Univ Paris Saclay, Inst Jean Pierre Bourgin, AgroParisTech, INRAE, F-78000 Versailles, France

[3] Wageningen Univ & Res, Biosci, NL-6708 PB Wageningen, Netherlands

[4] Wageningen Univ & Res, Lab Mol Biol, NL-6708 PB Wageningen, Netherlands

[5] King Abdullah Univ Sci & Technol, Biol & Environm Sci & Engn Div BESE, Darwin21, Thuwal 239556900, Saudi Arabia

[6] Univ Vienna, Max Perutz Labs, A-1030 Vienna, Austria

来源：

GENES | 2021年 / 12卷 / 05期

关键词：

environmental stress; seed development; site II element; HSFA2; TT2; MYB5-MBW complex; TRANSCRIPTION FACTORS; DOMAIN PROTEIN; EXPRESSION; TOLERANCE; GENE; ACCUMULATION; NETWORK; CROSSTALK; TTG1; A2;

D O I：

10.3390/genes12050746

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

HEAT SHOCK FACTOR A2 (HSFA2) is a regulator of multiple environmental stress responses required for stress acclimation. We analyzed HSFA2 co-regulated genes and identified 43 genes strongly co-regulated with HSFA2 during multiple stresses. Motif enrichment analysis revealed an over-representation of the site II element (SIIE) in the promoters of these genes. In a yeast 1-hybrid screen with the SIIE, we identified the closely related R2R3-MYB transcription factors TT2 and MYB5. We found overexpression of MYB5 or TT2 rendered plants heat stress tolerant. In contrast, tt2, myb5, and tt2/myb5 loss of function mutants showed heat stress hypersensitivity. Transient expression assays confirmed that MYB5 and TT2 can regulate the HSFA2 promoter together with the other members of the MBW complex, TT8 and TRANSPARENT TESTA GLABRA 1 (TTG1) and that the SIIE was involved in this regulation. Transcriptomic analysis revealed that TT2/MYB5 target promoters were enriched in SIIE. Overall, we report a new function of TT2 and MYB5 in stress resistance and a role in SIIE-mediated HSFA2 regulation.

引用

页数：18

共 34 条

[1] Leucoanthocyanidin Dioxygenase in Arabidopsis thaliana: Characterization of mutant alleles and regulation by MYB-BHLH-TTG1 transcription factor complexes [J].

Appelhagen, Ingo ;

Jahns, Oliver ;

Bartelniewoehner, Lutz ;

Sagasser, Martin ;

Weisshaar, Bernd ;

Stracke, Ralf .

GENE, 2011, 484 (1-2) :62-69

[2] MEME SUITE: tools for motif discovery and searching [J].

Bailey, Timothy L. ;

Boden, Mikael ;

Buske, Fabian A. ;

Frith, Martin ;

Grant, Charles E. ;

Clementi, Luca ;

Ren, Jingyuan ;

Li, Wilfred W. ;

Noble, William S. .

NUCLEIC ACIDS RESEARCH, 2009, 37 :W202-W208

[3] The Heat-Inducible Transcription Factor HsfA2 Enhances Anoxia Tolerance in Arabidopsis [J].

Banti, Valeria ;

Mafessoni, Fabrizio ;

Loreti, Elena ;

Alpi, Amedeo ;

Perata, Pierdomenico .

PLANT PHYSIOLOGY, 2010, 152 (03) :1471-1483

[4] TT8 controls its own expression in a feedback regulation involving TTG1 and homologous MYB and bHLH factors, allowing a strong and cell-specific accumulation of flavonoids in Arabidopsis thaliana [J].

Baudry, Antoine ;

Caboche, Michel ;

Lepiniec, Loic .

PLANT JOURNAL, 2006, 46 (05) :768-779

[5]

Bernard V, 2010, GENOME, V53, P739, DOI [10.1139/G10-042, 10.1139/g10-042]

[6] Speeding Cis-Trans Regulation Discovery by Phylogenomic Analyses Coupled with Screenings of an Arrayed Library of Arabidopsis Transcription Factors [J].

Castrillo, Gabriel ;

Turck, Franziska ;

Leveugle, Magalie ;

Lecharny, Alain ;

Carbonero, Pilar ;

Coupland, George ;

Paz-Ares, Javier ;

Onate-Sanchez, Luis .

PLOS ONE, 2011, 6 (06)

[7] The Effect of TRANSPARENT TESTA2 on Seed Fatty Acid Biosynthesis and Tolerance to Environmental Stresses during Young Seedling Establishment in Arabidopsis [J].

Chen, Mingxun ;

Wang, Zhong ;

Zhu, Yana ;

Li, Zhilan ;

Hussain, Nazim ;

Xuan, Lijie ;

Guo, Wanli ;

Zhang, Guoping ;

Jiang, Lixi .

PLANT PHYSIOLOGY, 2012, 160 (02) :1023-1036

[8] FORCA, a promoter element that responds to crosstalk between defense and light signaling [J].

Evrard, Alexandre ;

Ndatimana, Theogene ;

Eulgem, Thomas .

BMC PLANT BIOLOGY, 2009, 9

[9] The endogenous GL3, but not EGL3, gene is necessary for anthocyanin accumulation as induced by nitrogen depletion in Arabidopsis rosette stage leaves [J].

Feyissa, Dugassa N. ;

Lovdal, Trond ;

Olsen, Kristine M. ;

Slimestad, Rune ;

Lillo, Cathrine .

PLANTA, 2009, 230 (04) :747-754

[10] HsfA2 Controls the Activity of Developmentally and Stress-Regulated Heat Stress Protection Mechanisms in Tomato Male Reproductive Tissues [J].

Fragkostefanakis, Sotirios ;

Mesihovic, Anida ;

Simm, Stefan ;

Paupiere, Marine Josephine ;

Hu, Yangjie ;

Paul, Puneet ;

Mishra, Shravan Kumar ;

Tschiersch, Bettina ;

Theres, Klaus ;

Bovy, Arnaud ;

Schleiff, Enrico ;

Scharf, Klaus-Dieter .

PLANT PHYSIOLOGY, 2016, 170 (04) :2461-2477

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