Rapid PCR for identity testing using a battery-powered miniature thermal cycler

被引：0

作者：

Belgrader, P

Smith, JK

Weedn, VW

Northrup, MA

机构：

[1] Univ Calif Lawrence Livermore Natl Lab, Biol & Biotechnol Res Program, Livermore, CA 94550 USA

[2] Armed Forces Inst Pathol, DNA Identificat Lab, Rockville, MD 20850 USA

[3] Cepheid, Sunnyvale, CA 94089 USA

来源：

JOURNAL OF FORENSIC SCIENCES | 1998年 / 43卷 / 02期

关键词：

forensic science; rapid PCR; DNA typing; microchip;

D O I：

暂无

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

A microfabricated, battery-powered thermal cycler was implemented in PCR-based DNA typing for human identification. HLA DQ alpha and an STR tripler were PCR amplified using a device known as the Miniature Analytical Thermal Cycling Instrument (MATCI). The extremely efficient heating properties of the MATCI enabled thermal cycling to be completed in as little as 21 min. In addition, the feasibility of using the real-time fluorescent detection system of the MATCI was demonstrated. The successful application of this portable, prototype device to forensic identity testing is a significant milestone towards the eventual development of a completely integrated DNA testing instrument that would also incorporate sample preparation and allele detection.

引用

页码：315 / 319

页数：5