Sulfated glycosaminoglycans in bladder tissue and urine of rats after acute exposure to paraoxon and cyclophosphamide

被引:11
作者
Sobolev, Vladislav E. [1 ]
Jenkins, Richard O. [2 ]
Goncharov, Nikolay V. [1 ,3 ]
机构
[1] Res Inst Hyg Occupat Pathol & Human Ecol, St Petersburg 188663, Russia
[2] De Montfort Univ, Sch Allied Hlth Sci, Leicester LE1 9BH, Leics, England
[3] Russian Acad Sci, Sechenov Inst Evolutionary Physiol & Biochem, St Petersburg, Russia
基金
俄罗斯科学基金会;
关键词
Glycosaminoglycans; Paraoxon; Cyclophosphamide; Urinary bladder; Cystitis; HEPARAN-SULFATE; BASEMENT-MEMBRANE; DIMETHYLMETHYLENE BLUE; EXCRETION; WISTAR;
D O I
10.1016/j.etp.2017.02.007
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Glycosaminoglycans (GAGs) in the urine of Wistar rats, and on the surface of the epithelium and lamina propria of the bladder, were quantitatively assessed before and after acute intoxication with paraoxon or cyclophosphamide. Paraoxon was administered subcutaneously (s.c.) twice with an interval of 1 h to a final dose of 275 mg/kg; cyclophosphamide was administered intraperitoneally (i.p.) with a single dose of 100 mg/kg or to a final dose of 240 mg/kg (three times per 80 mg/kg every 12 h). GAGs on the surface of the epithelium and lamina propria of the urinary bladder of rats were quantitatively determined by Alcian blue dye staining. GAGs in the urine were determined spectrophotometrically with 1-9-dimethyl methylene blue. By 48-96 h after intoxication with either paraoxon or cyclophosphamide, statistically significant increases in the concentration of GAGs were obtained both for the tissues of the bladder and the urine of rats. Cyclophosphamide, in contrast to paraoxon, caused the development of hemorrhagic cystitis in the animals. The synthesis of GAGs is considered to be compensatory response to the toxic xenobiotics. (C) 2017 Elsevier GmbH. All rights reserved.
引用
收藏
页码:339 / 347
页数:9
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