ZNF552, a novel human KRAB/C2H2 zinc finger protein, inhibits AP-1-and SRE-mediated transcriptional activity

被引：6

作者：

Deng, Yun ^{[1
]}

Liu, Bisheng ^{[1
]}

Fan, Xiongwei ^{[1
]}

Wang, Yuequn ^{[1
]}

Tang, Ming ^{[1
]}

Mo, Xiaoyang ^{[1
]}

Li, Yongqing ^{[1
]}

Ying, Zaochu ^{[1
]}

Wan, Yongqi ^{[1
]}

Lao, Na ^{[1
]}

Zhou, Junmei ^{[1
]}

Wu, Xiushan ^{[1
]}

Yuan, Wuzhou ^{[1
]}

机构：

[1] Hunan Normal Univ, Coll Life Sci, Ctr Heart Dev, Key Lab MOE Dev Biol & Prot Chem, Changsha 410081, Hunan, Peoples R China

来源：

BMB REPORTS | 2010年 / 43卷 / 03期

基金：

中国国家自然科学基金;

关键词：

C2H2; KRAB; RNAi; Signal pathway; ZNF552; SIGNALING PATHWAY; CRYSTAL-STRUCTURE; DOMAIN; REPRESSION; FAMILY; COREPRESSOR; SEQUENCE; COMPLEX; KAP-1; CELLS;

D O I：

10.5483/BMBRep.2010.43.3.193

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, we report the identification and characterization of a novel C2H2 zinc finger protein, ZNF552, from a human embryonic heart cDNA library. ZNF552 is composed of three exons and two introns and maps to chromosome 19q13.43. The cDNA of ZNF552 is 2.3 kb, encoding 407 amino acids with an amino-terminal KRAB domain and seven carboxyl-terminal C2H2 zinc finger motifs in the nucleus and cytoplasm. Northern blotting analysis indicated that a 2.3 kb transcript specific for ZNF552 was expressed in liver, lung, spleen, testis and kidney, especially with a higher level in the lung and testis in human adult tissues. Reporter gene assays showed that ZNF552 was a transcriptional repressor, and overexpression of ZNF552 in the COS-7 cells inhibited the transcriptional activities of AP-1 and SRE, which could be relieved through RNAi analysis. Deletion studies showed that the KRAB domain of ZNF552 may be involved in this inhibition. [BMB reports 2010; 43(3): 193-198]

引用

页码：193 / 198

页数：6

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