A critical role of CDKN3 in gastric cancer and regulates tumor cell proliferation

The aim of this study was to clarify the role of cyclin-dependent kinase inhibitor 3 (CDKN3) in the development of gastric cancer. CDKN3 and functions as a key negative regulatory of cell cycle progression. Deregulation or mutations of CDKN3 have been implicated in various cancers. Small interfering RNA was used to silence CDKN3 expression in gastric cancer derived SGC-7901 cells. Proliferation, invasion and adhesion assays were done in engineered cells to assess the effect of CDKN3 silencing. Our data showed that CDKN3 loss dramatically decreased cell proliferation and significantly decreased cell invasive and adhesion potential. Additionally, in the limited set of genes whose expression status was determined CDKN3 loss was associated with decreased CDK1 and CDK2 expression. These results show that CDKN3 silenced in SGC-7901 cells are markedly influenced the genetic and phenotypic in gastric cancer, indicating that CDKN3 preferentially targets certain gene promoters.