Efficacy of cryopreservation of embryos generated by intracytoplasmic sperm injection with spermatozoa from frozen testicular tissue

被引:10
作者
Park, Yong-Seog [1 ,2 ]
Kim, Myo Kyung [1 ]
Lim, Chun Kyu [1 ]
Lee, Sun-Hee [1 ]
Park, Dong-Wook [1 ]
Seo, Ju Tae [1 ]
Yang, Kwang Moon [1 ]
机构
[1] Kwandong Univ, Coll Med, Cheil Gen Hosp & Womens Healthcare Ctr, Dept Obstet & Gynecol,Dept Urol,Lab Reprod Med, Seoul 100380, South Korea
[2] Kwandong Univ, Coll Med, Cheil Gen Hosp & Womens Healthcare Ctr, Lab Reprod Med, Seoul 100380, South Korea
关键词
Azoospermia; Frozen-thawed embryos; Frozen-thawed ET (FET); Frozen-thawed testicular sperm (t-TESE); Testicular sperm extraction (TESE); SURGICALLY RETRIEVED SPERM; AZOOSPERMIC MEN; PREGNANCY RATE; NONOBSTRUCTIVE AZOOSPERMIA; CLINICAL FACTORS; FERTILIZATION; FRESH; CYCLES; ICSI; OOCYTES;
D O I
10.1007/s10815-014-0304-2
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
To evaluate the viability of frozen embryos generated by intracytoplasmic sperm injection (ICSI) with frozen testicular spermatozoa. A total of 68 fresh embryo transfer (ET) cycles and 85 subsequent frozen-thawed ET (FET) cycles were grouped according to the source of spermatozoa: fresh testicular spermatozoa (TESE) or frozen-thawed testicular spermatozoa (t-TESE). There were no significant differences in the age of female patients, number of oocytes, or fertilization rates in fresh ET cycles with TESE (TESE-fresh ET) versus t-TESE (t-TESE-fresh ET). The rate of embryo survival after thawing (95.7 % vs. 94.0 %) was similar in frozen ET cycles (FET) with TESE (TESE-FET) and with t-TESE (t-TESE-FET). While there were significant differences in the proportion of good quality embryos, no statistical differences were found in the pregnancy or clinical abortion rates between the two groups. Moreover, delivery rates were not significantly different. Although the proportion of good quality embryos was affected by cryopreservation of testicular tissue, embryo survival rate was not. As well, subsequent pregnancy could be achieved successfully via t-TESE-FET cycles. Therefore, FET is not affected by the cryopreservation of testicular tissue, and avoids further oocyte retrieval and TESE procedures.
引用
收藏
页码:1331 / 1336
页数:6
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