Immunological proteo-glycolipidic interfacial film obtained from spreading of liposomes including ascitic fluid

This study focuses on the formation of an immunological proteo-glycolipidic interfacial film obtained from spreading of synthetic glycolipid liposomes including ascitic fluid. It is shown that the lipid-protein interactions created in liposomes before the monolayer formation are optimal to carry a large amount of proteinaceous material to the air-water interface. The strong amphipathic balance and the high fluidity of the hydrocarbon chains of the synthetic glycolipid used in this study are the two main properties enabling both retention of immunological proteins in the lipidic matrix and preservation of the structural organisation of the proteo-glycolipidic interfacial film during the transfer through vertical Langmuir-Blodgett deposition. (C) 2000 Elsevier Science B.V. All rights reserved.