Determination of enantiomeric purity of hyoscyamine from scopolia extract using HPLC-CD system without chiral separation

被引：0
|
作者：
Kudo, K
Iwaya, K
Yomota, C
Morris, S
Saito, M
机构：
[1] JASCO Inc, Easton, MD 21601 USA
[2] Natl Inst Hlth Sci, Chuo Ku, Osaka 540006, Japan
[3] JASCO Corp, Hachioji, Tokyo 1928537, Japan
来源：
ENANTIOMER | 2000年 / 5卷 / 3-4期
关键词：
scopolia; hyoscyamine; CD detector; circular dichroism; chiral; g-factor; EE%; enantiomeric analysis;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Enantiomeric ratio of hyoscyamine from Scopolia extract was determined by chiral HPLC-CD analysis. It was found that circular dichroism (CD) detection allowed the analysis of the sample without any special pretreatment whereas UV detection required an ammonia-ether extraction. To obtain a shorter analysis time for the determination, reversed-phase HPLC-CD analysis was applied by using a g-factor calibration curve (EE% vs. CD/UV). The analysis time was shortened from 35 to 18 min. EE% values obtained were consistent with those by chiral HPLC analysis.
引用
收藏
页码：369 / 375
页数：7
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