The pea mitochondrial nucleoside diphosphate kinase cleaves DNA and RNA

被引：27

作者：

Hammargren, Jenni

Salinas, Thalia

Marechal-Drouard, Laurence

Knorpp, Carina

机构：

[1] Swedish Univ Agr Sci, Dept Plant Biol & Forest Genet, S-75007 Uppsala, Sweden

[2] Univ Strasbourg, CNRS, Inst Biol Mol Plantes, Unite Propre Rech 2357, F-67084 Strasbourg, France

来源：

FEBS LETTERS | 2007年 / 581卷 / 18期

关键词：

NDPK; mitochondria; plant; intermembrane space; nuclease; tRNA;

D O I：

10.1016/j.febslet.2007.06.062

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Here, we present the characterization of a plant NDPK exhibiting nuclease activity. This is the first identification of a nuclease localised in the intermembrane space of plant mitochondria. The recombinant pea NDPK3 protein cleaves not only supercoiled plasmid DNA, but also highly structured RNA molecules such as tRNAs or the YUTR of the atp9 mRNA suggesting that the NDPK3 nuclease activity has a structural requirement. ATP inhibits this nuclease activity, while ADP has no effect. Furthermore, studies on NDPK mutant proteins indicate that the nuclease- and the kinase-mechanisms are separate. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

收藏

页码：3507 / 3511

页数：5

相关论文

共 37 条

[1] Solution structure of the biologically relevant g-quadruplex element in the human c-MYC promoter. implications for g-quadruplex stabilization [J].

Ambrus, A ;

Chen, D ;

Dai, JX ;

Jones, RA ;

Yang, DZ .

BIOCHEMISTRY, 2005, 44 (06) :2048-2058

[2] The intermembrane space of plant mitochondria contains a DNase activity that may be involved in programmed cell death [J].

Balk, J ;

Chew, SK ;

Leaver, CJ ;

McCabe, PF .

PLANT JOURNAL, 2003, 34 (05) :573-583

[3] Energy status and its control on embryogenesis of legumes:: ATP distribution within Vicia faba embryos is developmentally regulated and correlated with photosynthetic capacity [J].

Borisjuk, L ;

Rolletschek, H ;

Walenta, S ;

Panitz, R ;

Wobus, U ;

Weber, H .

PLANT JOURNAL, 2003, 36 (03) :318-329

[4] Phytochrome signalling is mediated through nucleoside diphosphate kinase 2 [J].

Choi, G ;

Yi, H ;

Lee, J ;

Kwon, YK ;

Soh, MS ;

Shin, BC ;

Luka, Z ;

Hahn, TR ;

Song, PS .

NATURE, 1999, 401 (6753) :610-613

[5] NDPK-A (but not NDPK-B) and AMPK α1 (but not AMPK α2) bind the cystic fibrosis transmembrane conductance regulator in epithelial cell membranes [J].

Crawford, Russell M. ;

Treharne, Kate J. ;

Best, O. Giles ;

Riemen, Claudia E. ;

Muimo, Richmond ;

Gruenert, Dieter C. ;

Arnaud-Dabernat, Sandrine ;

Daniel, Jean-Yves ;

Mehta, Anil .

CELLULAR SIGNALLING, 2006, 18 (10) :1595-1603

[6] In vitro import of a nuclearly encoded tRNA into mitochondria of Solanum tuberosum [J].

Delage, L ;

Dietrich, A ;

Cosset, A ;

Maréchal-Drouard, L .

MOLECULAR AND CELLULAR BIOLOGY, 2003, 23 (11) :4000-4012

[7] Tumor suppressor NM23-H1 is a granzyme A-activated DNase during CTL-mediated apoptosis, and the nucleosome assembly protein SET is its inhibitor [J].

Fan, ZS ;

Beresford, PJ ;

Oh, DY ;

Zhang, D ;

Lieberman, J .

CELL, 2003, 112 (05) :659-672

[8]

Farré EM, 2001, PLANT PHYSIOL, V127, P685, DOI 10.1104/pp.127.2.685

[9] Plant mitochondrial polyadenylated mRNAs are degraded by a 3′- to 5′-exoribonuclease activity, which proceeds unimpeded by stable secondary structures [J].

Gagliardi, D ;

Perrin, R ;

Maréchal-Drouard, L ;

Grienenberger, JM ;

Leaver, CJ .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (47) :43541-43547

[10] INFLUENCE OF PHOTORESPIRATION ON ATP/ADP RATIOS IN THE CHLOROPLASTS, MITOCHONDRIA, AND CYTOSOL, STUDIES BY RAPID FRACTIONATION OF BARLEY (HORDEUM-VULGARE) PROTOPLASTS [J].

GARDESTROM, P ;

WIGGE, B .

PLANT PHYSIOLOGY, 1988, 88 (01) :69-76

← 1 2 3 4 →