Integrated DNA Methylation and Gene Expression Analysis Identified S100A8 and S100A9 in the Pathogenesis of Obesity

被引：9

作者：

Chen, Ningyuan ^{[1
]}

Miao, Liu ^{[2
]}

Lin, Wei ^{[3
]}

Zou, Donghua ^{[4
]}

Huang, Ling ^{[1
]}

Huang, Jia ^{[5
]}

Shi, Wanxin ^{[5
]}

Li, Lilin ^{[5
]}

Luo, Yuxing ^{[5
]}

Liang, Hao ^{[5
]}

Pan, Shangling ^{[1
]}

Peng, Junhua ^{[1
]}

机构：

[1] Guangxi Med Univ, Sch Preclin Med, Dept Pathophysiol, Nanning, Peoples R China

[2] Guangxi Med Univ, Liuzhou Peoples Hosp, Dept Cardiol, Liuzhou, Peoples R China

[3] Guangxi Jiangbin Hosp, Dept Neurol Rehabil, Nanning, Peoples R China

[4] Guangxi Med Univ, Affiliated Hosp 1, Dept Neurol, Nanning, Peoples R China

[5] Guangxi Med Univ, Clin Med Sch 1, Nanning, Peoples R China

来源：

FRONTIERS IN CARDIOVASCULAR MEDICINE | 2021年 / 8卷

基金：

中国国家自然科学基金;

关键词：

obesity; DNA methylation; mRNA expression; function enrichment; correlation analyses; BODY-MASS INDEX; EPIGENOME-WIDE ASSOCIATION; GASTRIC BYPASS; OVERWEIGHT; PROTEIN; CALPROTECTIN; DISEASE;

D O I：

10.3389/fcvm.2021.631650

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Background: To explore the association of DNA methylation and gene expression in the pathology of obesity. Methods: (1) Genomic DNA methylation and mRNA expression profile of visceral adipose tissue (VAT) were performed in a comprehensive database of gene expression in obese and normal subjects. (2) Functional enrichment analysis and construction of differential methylation gene regulatory networks were performed. (3) Validation of the two different methylation sites and corresponding gene expression was done in a separate microarray dataset. (4) Correlation analysis was performed on DNA methylation and mRNA expression data. Results: A total of 77 differentially expressed mRNAs matched with differentially methylated genes. Analysis revealed two different methylation sites corresponding to two unique genes-s100a8-cg09174555 and s100a9-cg03165378. Through the verification test of two interesting different expression positions [differentially methylated positions (DMPs)] and their corresponding gene expression, we found that methylation in these genes was negatively correlated to gene expression in the obesity group. Higher S100A8 and S100A9 expressions in obese subjects were validated in a separate microarray dataset. Conclusion: This study confirmed the relationship between DNA methylation and gene expression and emphasized the important role of S100A8 and S100A9 in the pathogenesis of obesity.

引用

页数：13

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