Direct detection of Streptococcus pneumoniae in positive blood cultures by real-time polymerase chain reaction

被引：9
|
作者：
Selva, Laura [1 ]
Esteva, Cristina [1 ]
Gene, Amadeu [1 ]
Fernandez de Sevilla, Maria [2 ]
Hernandez-Bou, Susanna [2 ]
Munoz-Almagro, Carmen [1 ]
机构：
[1] Univ Hosp St Joan de Deu, Dept Mol Microbiol, Esplugues Barcelona 08950, Spain
[2] Univ Hosp St Joan de Deu, Dept Paediat, Esplugues Barcelona 08950, Spain
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2010年 / 66卷 / 02期
关键词：
Real-time PCR; Blood culture bottles; Streptococcus pneumoniae; COMMUNITY-ACQUIRED PNEUMONIA; RAPID DETECTION; PCR; BOTTLES; IDENTIFICATION; CHILDREN; DNA;
D O I：
10.1016/j.diagmicrobio.2009.05.023
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
We developed a real-time polymerase chain reaction specific for Streptococcus pneumoniae to be applied directly from blood culture bottles without previous DNA extraction step. For the 128 blood culture bottles tested, the assay had 94% and 98.4% sensitivity and specificity, respectively. This assay provides rapid and accurate identification of this pathogen. (C) 2010 Elsevier Inc. All rights reserved.
引用
收藏
页码：204 / 206
页数：3
相关论文
共 50 条
  • [41] Development of Streptococcus gordonii-specific quantitative real-time polymerase chain reaction primers based on the nucleotide sequence of rpoB
    Park, Soon-Nang
    Kook, Joong-Ki
    MICROBIOLOGY AND IMMUNOLOGY, 2013, 57 (08) : 583 - 588
  • [42] Real-Time Assay as A Tool for Detecting lytA Gene in Streptococcus pneumoniae Isolates
    Hajia, Masoud
    Farzanehkhah, Mohammad
    Hajihashemi, Bahareh
    Dolatyar, AliReza
    Imani, Mohsen
    Saburian, Roghieh
    Farzami, Marjan Rahnamaye
    Rahbar, Mohammad
    CELL JOURNAL, 2014, 16 (02) : 141 - 146
  • [43] Real-Time Polymerase Chain Reaction MicroRNA Detection Based on Enzymatic Stem-Loop Probes Ligation
    Li, Juan
    Yao, Bo
    Huang, Huang
    Wang, Zhao
    Sun, Changhong
    Fan, Yu
    Chang, Qing
    Li, Shaolu
    Wang, Xiang
    Xi, Jianzhong
    ANALYTICAL CHEMISTRY, 2009, 81 (13) : 5446 - 5451
  • [44] Sensitive Detection of Soy (Glycine max) by Real-Time Polymerase Chain Reaction Targeting the Mitochondrial atpA Gene
    Bauer, Tobias
    Kirschbaum, Katja
    Panter, Silvia
    Kenk, Marion
    Bergemann, Joerg
    JOURNAL OF AOAC INTERNATIONAL, 2011, 94 (06) : 1863 - 1873
  • [45] Development of a diagnostic real-time polymerase chain reaction assay for the detection of invasive Haemophilus influenzae in clinical samples
    Meyler, Kenneth L.
    Meehan, Mary
    Bennett, Desiree
    Cunney, Robert
    Cafferkey, Mary
    DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2012, 74 (04) : 356 - 362
  • [46] An improved real-time polymerase chain reaction for the simultaneous detection of all serotypes of Epizootic hemorrhagic disease virus
    Clavijo, Alfonso
    Sun, Feng
    Lester, Thomas
    Jasperson, Dane C.
    Wilson, William C.
    JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 2010, 22 (04) : 588 - 593
  • [47] Diagnostic performance of real-time polymerase chain reaction assay on blood for invasive aspergillosis and mucormycosis
    Seo, Hyeonji
    Kim, Ji Yeun
    Son, Hyo-Ju
    Jung, Jiwon
    Kim, Min Jae
    Chong, Yong Pil
    Lee, Sang-Oh
    Choi, Sang-Ho
    Kim, Yang Soo
    Kim, Sung-Han
    MYCOSES, 2021, 64 (12) : 1554 - 1562
  • [48] The diagnostic value of serological tests and real-time polymerase chain reaction in children with acute Mycoplasma pneumoniae infection
    Li, Qing-Ling
    Dong, He-Ting
    Sun, Hui-Ming
    Zhang, Xin-Xing
    Gu, Wen-Jing
    Huang, Li
    Wang, Yu-Qing
    Yan, Yong-Dong
    Ji, Wei
    Hao, Chuang-Li
    Zhu, Can-Hong
    Chen, Zheng-Rong
    ANNALS OF TRANSLATIONAL MEDICINE, 2020, 8 (06)
  • [49] Evaluation of the polymerase chain reaction method for detection of Streptococcus pneumoniae DNA in pleural fluid samples
    Falguera, M
    López, A
    Nogués, A
    Porcel, JM
    Rubio-Caballero, M
    CHEST, 2002, 122 (06) : 2212 - 2216
  • [50] Clinical Impact of Pleural Fluid Streptococcus pneumoniae Polymerase Chain Reaction Testing in Children With Complicated Pneumonia
    Ho, Erin C.
    Olson, Kaitlin E.
    Butler, Molly
    Birkholz, Meghan
    Miller, Kristen
    MacBrayne, Christine E.
    Jung, Sarah
    Messacar, Kevin
    Asturias, Edwin J.
    Dominguez, Samuel R.
    CLINICAL INFECTIOUS DISEASES, 2024, 79 (06) : 1487 - 1494
12345