PATTERN OF MATRIX METALLOPROTEINASES-9, P53 AND BCL-2 PROTEINS IN EGYPTIAN PATIENTS WITH PULMONARY MYCOBACTERIUM TUBERCULOSIS

被引:6
作者
El-Masry, Samir [1 ]
Lotfy, Mahmoud [1 ]
Samy, Mona [2 ]
Moawia, Shadin [1 ]
El-Sayed, Ibrahim H. [1 ]
Khamees, Islam M. [1 ]
机构
[1] Menoufia Univ, Genet Engn & Biotechnol Res Inst, Dept Mol & Cellular Biol, Sadat City, Minufiya, Egypt
[2] Mansoura Univ, Children Hosp, Mansoura, Egypt
关键词
Mycobacterium tuberculosis; matrix metalloproteinases; P53; Bcl-2; HUMAN MONOCYTIC CELLS; GELATINOLYTIC ACTIVITY; TISSUE INHIBITOR; APOPTOSIS; MACROPHAGES; HOST; EXPRESSION; INDUCTION; RELEASE; MATRIX-METALLOPROTEINASE-9;
D O I
10.1556/AMicr.57.2010.2.5
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Matrix metalloproteinases (MMPs) constitute a large family of enzymes that degrade extracellular matrix proteins (ECM). MMPs are implicated in different pathological conditions such as cancer. Bcl-2 and P53 are key controllers of programmed cell death (PCD) or apoptosis. The aim of the present study was to determine the MMP-9, P53 and Bcl-2 levels in Egyptian patients with Mycobacterium tuberculosis (MTB) (Group I) compared with healthy control individuals (Group II). The concentrations of serum MMP-9 were determined quantitatively using enzyme immunoassay (EIA). P53 and Bcl-2 levels were assayed by flow cytometric analysis using specific monoclones. MMP-9 level was significantly higher in MTB patients compared with healthy control. Similarly, P53 and Bcl-2 levels were increased in MTB patients compared with healthy ones. These data reflect the alteration of MMP-9 level during the course of MTB infection, accompanied with apparent dysregulation of cellular apoptosis as indicated by P53 and Bcl-2 over-expression.
引用
收藏
页码:123 / 133
页数:11
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