Structural characterization of N-glycans of cauxin by MALDI-TOF mass spectrometry and nano LC-ESI-mass spectrometry

被引：5

作者：

Suzuki, Yusuke

Miyazaki, Masao

Ito, Emi

Suzuki, Minoru

Yamashita, Tetsuro

Taira, Hideharu

Suzuki, Akemi

机构：

[1] RIKEN, Frontier Res Syst, Supramol Syst Res Grp, Wako, Saitama 3510198, Japan

[2] RIKEN, Ctr Intellectural Property Strategies, Appl Mass Spectrometry Lab, Wako, Saitama 3510198, Japan

[3] Iwate Univ, Fac Agr, Dept Agrobiosci, Morioka, Iwate 0208550, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2007年 / 71卷 / 03期

基金：

日本科学技术振兴机构;

关键词：

cauxin; glycoproteins; N-glycan; mass spectrometry;

D O I：

10.1271/bbb.60599

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cauxin is a carboxylesterase-like glycoprotein excreted as a major component of cat urine. Cauxin contains four putative N-glycosylation sites. We characterized the structure of an N-linked oligosaccharide of cauxin using nano liquid chromatography (LC)-electrospray ionization (ESI) and matrix-assisted laser desorption/ ionization quadrupole ion trap time-of-flight mass spectrometry (MALDI-QIT-TOF MS) and MS/MS, and high-performance liquid chromatography (HPLC) with an octadecylsilica (ODS) column. The structure of the N-linked oligosaccharide of cauxin attached to (83)Asn was a bisecting complex type, Gal beta 1-4GlcNAc beta 1-Man alpha 1-3(Gal beta 1-4GlcNAc beta 1-2Man alpha 1-6)(GlcNAc beta 1-4)- 2Man alpha 1-4GlcNAc beta 1-4(Fuc alpha 1-6)GlcNAc.

引用

页码：811 / 816

页数：6

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