Tricistronic and tetracistronic retroviral vectors for gene transfer

被引：29

作者：

De Felipe, P ^{[1
]}

Izquierdo, M ^{[1
]}

机构：

[1] Univ Autonoma Madrid, Fac Ciencias, Ctr Biol Mol Severo Ochoa, Dept Bioquim & Biol Mol, E-28049 Madrid, Spain

来源：

HUMAN GENE THERAPY | 2000年 / 11卷 / 13期

关键词：

D O I：

10.1089/10430340050129530

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have combined the picornavirus foot-and-mouth disease virus (FMDV) 2A sequence and the internal ribosome entry sites (IRESes) from encephalomyocarditis virus (ECMV) and avian reticuloendotheliosis virus type A (REV-A) to construct tricistronic and tetracistronic vectors. All the polycistronic constructs show high titers and expression of the genes inserted. Clones have been obtained in which cells simultaneously express the three or four genes carried by the polycistronic vectors.

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页码：1921 / 1931

页数：11

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