Tricistronic and tetracistronic retroviral vectors for gene transfer

被引：29

作者：

De Felipe, P ^{[1
]}

Izquierdo, M ^{[1
]}

机构：

[1] Univ Autonoma Madrid, Fac Ciencias, Ctr Biol Mol Severo Ochoa, Dept Bioquim & Biol Mol, E-28049 Madrid, Spain

来源：

HUMAN GENE THERAPY | 2000年 / 11卷 / 13期

关键词：

D O I：

10.1089/10430340050129530

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have combined the picornavirus foot-and-mouth disease virus (FMDV) 2A sequence and the internal ribosome entry sites (IRESes) from encephalomyocarditis virus (ECMV) and avian reticuloendotheliosis virus type A (REV-A) to construct tricistronic and tetracistronic vectors. All the polycistronic constructs show high titers and expression of the genes inserted. Clones have been obtained in which cells simultaneously express the three or four genes carried by the polycistronic vectors.

引用

页码：1921 / 1931

页数：11

共 54 条

[31] MILLER AD, 1989, BIOTECHNIQUES, V7, P980
[32] INSERTION OF SEVERAL DIFFERENT DNAS IN RETICULOENDOTHELIOSIS VIRUS STRAIN-T SUPPRESSES TRANSFORMATION BY REDUCING THE AMOUNT OF SUBGENOMIC MESSENGER-RNA
MILLER, CK
TEMIN, HM
[J]. JOURNAL OF VIROLOGY, 1986, 58 (01) : 75 - 80
[33] RETROVIRAL VECTORS CONTAINING PUTATIVE INTERNAL RIBOSOME ENTRY SITES - DEVELOPMENT OF A POLYCISTRONIC GENE-TRANSFER SYSTEM AND APPLICATIONS TO HUMAN GENE-THERAPY
MORGAN, RA
COUTURE, L
ELROYSTEIN, O
RAGHEB, J
MOSS, B
ANDERSON, WF
[J]. NUCLEIC ACIDS RESEARCH, 1992, 20 (06) : 1293 - 1299
[34] ADVANCED MAMMALIAN GENE-TRANSFER - HIGH TITER RETROVIRAL VECTORS WITH MULTIPLE-DRUG SELECTION MARKERS AND A COMPLEMENTARY HELPER-FREE PACKAGING CELL-LINE
MORGENSTERN, JP
LAND, H
[J]. NUCLEIC ACIDS RESEARCH, 1990, 18 (12) : 3587 - 3596
[35] Moriuchi S, 1998, CANCER RES, V58, P5731
[36] Okada H, 1996, GENE THER, V3, P957
[37] STABLY TRANSMITTED TRIPLE-PROMOTER RETROVIRAL VECTORS AND THEIR USE IN TRANSFORMATION OF PRIMARY MAMMALIAN-CELLS
OVERELL, RW
WEISSER, KE
COSMAN, D
[J]. MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (04) : 1803 - 1808
[38] BROAD-SPECTRUM OF INVIVO FORWARD MUTATIONS, HYPERMUTATIONS, AND MUTATIONAL HOTSPOTS IN A RETROVIRAL SHUTTLE VECTOR AFTER A SINGLE REPLICATION CYCLE - SUBSTITUTIONS, FRAMESHIFTS, AND HYPERMUTATIONS
PATHAK, VK
TEMIN, HM
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (16) : 6019 - 6023
[39] Self-contained, tetracycline-regulated retroviral vector system for gene delivery to mammalian cells
Paulus, W
Baur, I
Boyce, FM
Breakefield, XO
Reeves, SA
[J]. JOURNAL OF VIROLOGY, 1996, 70 (01) : 62 - 67
[40] Production and characterization of a bicistronic Moloney-based retroviral vector expressing human interleukin 2 and herpes simplex virus thymidine kinase for gene therapy of cancer
Pizzato, M
Franchin, E
Calvi, P
Boschetto, R
Colombo, M
Ferrini, S
Palu, G
[J]. GENE THERAPY, 1998, 5 (07) : 1003 - 1007

← 1 2 3 4 5 6 →