Development of a high-throughput assay for the HIV-1 integrase disintegration reaction

被引：5

作者：

He HongQiu ^{[1
]}

Liu Bin ^{[1
]}

Zhang XiaoYi ^{[1
]}

Chen WeiZu ^{[1
]}

Wang CunXin ^{[1
]}

机构：

[1] Beijing Univ Technol, Coll Life Sci & Bioengn, Beijing 100124, Peoples R China

来源：

SCIENCE CHINA-LIFE SCIENCES | 2010年 / 53卷 / 02期

基金：

北京市自然科学基金; 中国国家自然科学基金;

关键词：

HIV-1; integrase; IN-CCD; disintegration; high-throughput assay; IMMUNODEFICIENCY-VIRUS TYPE-1; IN-VITRO; CATALYTIC DOMAIN; STRAND TRANSFER; IDENTIFICATION; PROTEIN; MUTANT; INHIBITION; BINDING;

D O I：

10.1007/s11427-010-0006-7

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Both HIV-1 integrase (IN) and the central catalytic domain of IN (IN-CCD) catalyze the disintegration reaction in vitro. In this study, IN and IN-CCD proteins were expressed and purified, and a high-throughput format enzyme-linked immunosorbent assay (ELISA) was developed for the disintegration reaction. IN exhibited a marked preference for Mn2+ over Mg2+ as the divalent cation cofactor in disintegration. Baicalein, a known IN inhibitor, was found to be an IN-CCD inhibitor. The assay is sensitive and specific for the study of disintegration reaction as well as for the in vitro identification of antiviral drugs targeting IN, especially targeting IN-CCD.

引用

页码：241 / 247

页数：7

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