Microbiological Sampling of Carcasses by Excision or Swabbing with Three Types of Sponge or Gauze

被引:22
作者
Martinez, Bernardo [3 ]
Felicidad Celda, M. [3 ]
Anastasio, Begona [3 ]
Garcia, Ines [2 ]
Carmen Lopez-Mendoza, M. [1 ]
机构
[1] Cardenal Herrera CEU Univ, Anim Prod & Food Sci & Technol Dept, E-46113 Moncada, Valencia, Spain
[2] INCA Lab, E-46910 Valencia, Spain
[3] Conselleria Sanidad, Ctr Publ Hlth Alzira, E-46600 Valencia, Spain
关键词
RED-MEAT; BEEF CARCASSES; CONTAMINATION; ABATTOIRS; QUALITY; PORK; 2001/471/EC; PATHOGENS; SURFACES; DECISION;
D O I
10.4315/0362-028X-73.1.81
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Fifty-five bovine, 50 equine, 60 ovine, and 50 porcine carcasses were sampled in a slaughterhouse in eastern Spain. Two samples were taken from each carcass, one using the excision method and the other using the swabbing method. Four different materials were used for swabbing: cellulose, polyurethane, or viscose sponges, and medical gauze. Samples were collected at the end of the process by four different people before the carcasses were taken to the cooler. The samples were examined for total viable bacteria counts (TVCs) and Enterobacteriaceae counts (ECs). The mean TVC for all species sampled by excision was 4.50 log CFU/cm(2) which was significantly higher than the 3.53 log CFU/cm(2) obtained by swabbing. The TVCs obtained using gauze and the cellulose and polyurethane sponges were significantly higher (P < 0.05) than the corresponding TVCs obtained using viscose sponges. Animal species, the person who collected the samples, and microbiological load also had a significant effect on TVC. ECs were obtained from 82.8% of excision samples, from larger percentages of samples obtained using cellulose or polyurethane sponges or gauze swabs, but from smaller percentages of samples obtained using viscose sponges. The Enterobacteriaceae load significantly influenced the EC. In contrast, animal species and the person who collected the samples had no significant effect. The cellulose sponge, polyurethane sponge, and gauze gave high mean log counts of aerobic bacteria and Enterobacteriaceae, which makes these swab types suitable for use in slaughterhouses for the purpose of assessing production process hygiene.
引用
收藏
页码:81 / 87
页数:7
相关论文
共 29 条
[1]  
[Anonymous], 2005, OFFICIAL J EUROPEAN
[2]   The EC decision of the 8th June 2001 (EC/471/2001): excision versus swabbing [J].
Bolton, DJ .
FOOD CONTROL, 2003, 14 (03) :207-209
[3]  
Bryant J, 2003, CAN VET J, V44, P51
[4]   The development of a 'clean sheep policy' in compliance with the new Hygiene Regulation (EC) 853/2004 (Hygiene 2) [J].
Byrne, B. ;
Dunne, G. ;
Lyng, J. ;
Bolton, D. J. .
FOOD MICROBIOLOGY, 2007, 24 (03) :301-304
[5]   Microbiological carcass sampling methods to achieve compliance with 2001/471/EC and new hygiene regulations [J].
Byrne, B ;
Dunne, G ;
Lyng, J ;
Bolton, DJ .
RESEARCH IN MICROBIOLOGY, 2005, 156 (01) :104-106
[6]   Sampling methods for microbiological analysis of red meat and poultry carcasses [J].
Capita, R ;
Prieto, M ;
Alonso-Calleja, C .
JOURNAL OF FOOD PROTECTION, 2004, 67 (06) :1303-1308
[7]   An in vitro system for the comparison of excision and wet-dry swabbing for microbiological sampling of beef carcasses [J].
Cenci-Goga, B. T. ;
Miraglia, D. ;
Ranucci, D. ;
Branciari, R. ;
Budelli, L. ;
McCrindle, C. M. ;
Cioffi, A. ;
Mammoli, R. .
JOURNAL OF FOOD PROTECTION, 2007, 70 (04) :930-936
[8]   Evaluation of six sampling methods for recovery of bacteria from beef carcass surfaces [J].
Dorsa, WJ ;
Cutter, CN ;
Siragusa, GR .
LETTERS IN APPLIED MICROBIOLOGY, 1996, 22 (01) :39-41
[9]   Comparison of swabbing and destructive methods for microbiological pig carcass sampling [J].
Ghafir, Y. ;
Daube, G. .
LETTERS IN APPLIED MICROBIOLOGY, 2008, 47 (04) :322-326
[10]   Hygiene indicator microorganisms for selected pathogens on beef, pork, and poultry meats in Belgium [J].
Ghafir, Y. ;
China, B. ;
Dierick, K. ;
De Zutter, L. ;
Daube, G. .
JOURNAL OF FOOD PROTECTION, 2008, 71 (01) :35-45