Aeromicrobium camelliae sp nov., isolated from Pu'er tea

A novel Gram-reaction-positive, aerobic and non-spore-forming rod-shaped bacterial strain, YS17(T), was isolated from ripened Pu'er tea. Growth of the strain was observed at 15-50 degrees C (optimum 30-37 degrees C) and at pH 5.5-10.5 (optimum 6.0-9.5). Phylogenetic analysis of 16S rRNA gene sequences indicated that the strain represented a member of the genus Aeromicrobium. The strains most closely related to YS17(T) were Aeromicrobium erythreum DSM 8599(T), Aeromicrobium alkaliterrae JCM 13518(T) and Aeromicrobium ginsengisoli JCM 14732(T), with 16S rRNA gene sequence similarities of 96.8, 96.8 and 96.7%, respectively. DNA-DNA hybridization of YS17(T) with the type strains of the most closely related species, A. erythreum DSM 8599(T), A. alkaliterrae JCM 13518(T) and A. ginsengisoli JCM 14732(T), yielded reassociation values of 10.9, 16.8 and 10.9%, respectively. The diagnostic diamino acid of the cell wall peptidoglycan was LL-diaminopimelic acid. The predominant menaquinones were menaquinone MK-9(H-4) (76 %) and MK-8(H-4) (17%). The major fatty acids were C-16 : 0, 10-methyl C-18:0 and C-18:1 omega 9c. The DNA G+C content of YS17(T) was 66 mol%. YS17(T) could be differentiated from recognized species of the genus Aeromicrobium on the basis of phenotypic characteristics, chemotaxonomic differences, phylogenetic analysis and DNA-DNA hybridization data. On the basis of evidence from the polyphasic analyses performed as part of this study a novel species, Aeromicrobium camelliae sp. nov., is proposed, with strain YS17(T) (=CGMCC 1.12942(T)=JCM 30952(T)) as the type strain.