Simulating distal gut mucosal and luminal communities using packed-column biofilm reactors and an in vitro chemostat model

被引：49

作者：

McDonald, Julie A. K. ^{[1
]}

Fuentes, Susana ^{[2
]}

Schroeter, Kathleen ^{[1
]}

Heikamp-deJong, Ineke ^{[2
]}

Khursigara, Cezar M. ^{[1
]}

de Vos, Willem M. ^{[2
]}

Allen-Vercoe, Emma ^{[1
]}

机构：

[1] Univ Guelph, Dept Mol & Cellular Biol, Guelph, ON N1G 2W1, Canada

[2] Wageningen Univ, Microbiol Lab, NL-6700 AP Wageningen, Netherlands

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2015年 / 108卷

基金：

加拿大健康研究院;

关键词：

Biofilm; Chemostat; DGGE; HITChip; Human gut microbiota; Microbial ecology; HUMAN INTESTINAL BACTERIA; MICROBIAL COMMUNITY; MOTILE BACTERIA; MUCIN; CLINDAMYCIN; DIVERSITY; COLONIZATION; RESISTANCE; STABILITY; ASSOCIATION;

D O I：

10.1016/j.mimet.2014.11.007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In vivo studies of human mucosal gut microbiota are often limited to end-point analyses and confounded by bowel cleansing procedures. Therefore, we used biofilm reactors to incorporate a simulated mucosal environment into an in vitro gut chemostat model. Communities developed were complex, reproducible, distinct, and representative of in vivo communities. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：36 / 44

页数：9

共 60 条

[1]

Allen-Vercoe E, 2013, EXPERT REV GASTROENT, V7, P291, DOI [10.1586/EGH.13.16, 10.1586/egh.13.16]

[2] STUDIES ON MIXED POPULATIONS OF HUMAN INTESTINAL BACTERIA GROWN IN SINGLE-STAGE AND MULTISTAGE CONTINUOUS CULTURE SYSTEMS [J].

ALLISON, C ;

MCFARLAN, C ;

MACFARLANE, GT .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1989, 55 (03) :672-678

[3] INTERACTION OF BIOFILM BACTERIA WITH ANTIBIOTICS IN A NOVEL INVITRO CHEMOSTAT SYSTEM [J].

ANWAR, H ;

VANBIESEN, T ;

DASGUPTA, M ;

LAM, K ;

COSTERTON, JW .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1989, 33 (10) :1824-1826

[4] DYNAMIC INTERACTIONS OF BIOFILMS OF MUCOID PSEUDOMONAS-AERUGINOSA WITH TOBRAMYCIN AND PIPERACILLIN [J].

ANWAR, H ;

STRAP, JL ;

CHEN, K ;

COSTERTON, JW .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1992, 36 (06) :1208-1214

[5] Host-bacterial mutualism in the human intestine [J].

Bäckhed, F ;

Ley, RE ;

Sonnenburg, JL ;

Peterson, DA ;

Gordon, JI .

SCIENCE, 2005, 307 (5717) :1915-1920

[6] Microbes inside-from diversity to function: the case of Akkermansia [J].

Belzer, Clara ;

de Vos, Willem M. .

ISME JOURNAL, 2012, 6 (08) :1449-1458

[7] High temporal and inter-individual variation detected in the human ileal microbiota [J].

Booijink, Carien C. G. M. ;

El-Aidy, Sahar ;

Rajilic-Stojanovic, Mirjana ;

Heilig, Hans G. H. J. ;

Troost, Freddy J. ;

Smidt, Hauke ;

Kleerebezem, Michiel ;

de Vos, Willem M. ;

Zoetendal, Erwin G. .

ENVIRONMENTAL MICROBIOLOGY, 2010, 12 (12) :3213-3227

[8] Profound Alterations of Intestinal Microbiota following a Single Dose of Clindamycin Results in Sustained Susceptibility to Clostridium difficile-Induced Colitis [J].

Buffie, Charlie G. ;

Jarchum, Irene ;

Equinda, Michele ;

Lipuma, Lauren ;

Gobourne, Asia ;

Viale, Agnes ;

Ubeda, Carles ;

Xavier, Joao ;

Pamer, Eric G. .

INFECTION AND IMMUNITY, 2012, 80 (01) :62-73

[9] MICROBIAL BIOFILMS [J].

COSTERTON, JW ;

LEWANDOWSKI, Z ;

CALDWELL, DE ;

KORBER, DR ;

LAPPINSCOTT, HM .

ANNUAL REVIEW OF MICROBIOLOGY, 1995, 49 :711-745

[10] Akkermansia muciniphila gen. nov., sp nov., a human intestinal mucin-degrading bacterium [J].

Derrien, M ;

Vaughan, EE ;

Plugge, CM ;

de Vos, WM .

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2004, 54 :1469-1476

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