Development of a Bioorthogonal and Highly Efficient Conjugation Method for Quantum Dots Using Tetrazine-Norbornene Cycloaddition

被引：182

作者：

Han, Hee-Sun ^{[1
]}

Devaraj, Neal K. ^{[2
]}

Lee, Jungmin ^{[1
]}

Hilderbrand, Scott A. ^{[2
]}

Weissleder, Ralph ^{[2
]}

Bawendi, Moungi G. ^{[1
]}

机构：

[1] MIT, Dept Chem, Cambridge, MA 02139 USA

[2] Massachusetts Gen Hosp, Ctr Syst Biol, Boston, MA 02114 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2010年 / 132卷 / 23期

关键词：

CLICK CHEMISTRY;

D O I：

10.1021/ja101677r

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We present a bioorthogonal and modular conjugation method for efficient coupling of organic dyes and biomolecules to quantum dots (QDs) using a norbornene-tetrazine cycloaddition. The use of noncoordinating functional groups combined with the rapid rate of the cycloaddition leads to highly efficient conjugation. We have applied this method to the in situ targeting of norbornene-coated QDs to live cancer cells labeled with tetrazine-modified proteins.

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收藏

页码：7838 / +

页数：3

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