Comparison of the in-house made Carba-NP and Blue-Carba tests: Considerations for better detection of carbapenemase-producing Enterobacteriaceae

被引:19
作者
Pires, Joao [1 ,2 ]
Tinguely, Regula [1 ]
Thomas, Bodmer [3 ]
Luzzaro, Francesco [4 ]
Endimiani, Andrea [1 ]
机构
[1] Univ Bern, Inst Infect Dis, Friedbuhlstr 51, CH-3010 Bern, Switzerland
[2] Univ Bern, Grad Sch Cellular & Biomed Sci, CH-3010 Bern, Switzerland
[3] Lab Med Zentrum Dr Risch, Dept Med Microbiol, Bern, Switzerland
[4] A Manzoni Hosp, Microbiol Lab, Lecce, Italy
基金
瑞士国家科学基金会;
关键词
Carbapenemases; Carbapenems; Diagnostics; MIC; Klebsiella; OXA-48; NDM; VIM; MBL; RESISTANT KLEBSIELLA-PNEUMONIAE; GRAM-NEGATIVE BACILLI; RAPID DETECTION; OXA-48; CARBAPENEMASE; SWITZERLAND; INFECTION; ISOLATE; KIT;
D O I
10.1016/j.mimet.2016.01.002
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The in-house Carba-NP and Blue-Carba tests were compared using 30 carbapenemase- and 33 non-producing Enterobacteriaceae. Tests were read by three operators. 100% sensitivity was reported for both tests, but Carba-NP was slightly more specific than Blue-Carba (98.9% vs. 91.7%). We describe potential sources of error during tests' preparation and reading. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码:33 / 37
页数:5
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