Comparison of the in-house made Carba-NP and Blue-Carba tests: Considerations for better detection of carbapenemase-producing Enterobacteriaceae

被引:19
作者
Pires, Joao [1 ,2 ]
Tinguely, Regula [1 ]
Thomas, Bodmer [3 ]
Luzzaro, Francesco [4 ]
Endimiani, Andrea [1 ]
机构
[1] Univ Bern, Inst Infect Dis, Friedbuhlstr 51, CH-3010 Bern, Switzerland
[2] Univ Bern, Grad Sch Cellular & Biomed Sci, CH-3010 Bern, Switzerland
[3] Lab Med Zentrum Dr Risch, Dept Med Microbiol, Bern, Switzerland
[4] A Manzoni Hosp, Microbiol Lab, Lecce, Italy
基金
瑞士国家科学基金会;
关键词
Carbapenemases; Carbapenems; Diagnostics; MIC; Klebsiella; OXA-48; NDM; VIM; MBL; RESISTANT KLEBSIELLA-PNEUMONIAE; GRAM-NEGATIVE BACILLI; RAPID DETECTION; OXA-48; CARBAPENEMASE; SWITZERLAND; INFECTION; ISOLATE; KIT;
D O I
10.1016/j.mimet.2016.01.002
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The in-house Carba-NP and Blue-Carba tests were compared using 30 carbapenemase- and 33 non-producing Enterobacteriaceae. Tests were read by three operators. 100% sensitivity was reported for both tests, but Carba-NP was slightly more specific than Blue-Carba (98.9% vs. 91.7%). We describe potential sources of error during tests' preparation and reading. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码:33 / 37
页数:5
相关论文
共 28 条
  • [21] Comparison of BD MAX Check-Points CPO assay with Cepheid Xpert Carba-R assay for the detection of carbapenemase-producing Enterobacteriaceae directly from rectal swabs
    Yoo, In Young
    Shin, Dong Pil
    Heo, Woong
    Ha, Sung Il
    Cha, Young Jong
    Park, Yeon-Joon
    DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2022, 103 (03)
  • [22] MALDI-TOF MS detection of carbapenemase activity in clinical isolates of Enterobacteriaceae spp., Pseudomonas aeruginosa, and Acinetobacter baumannii compared against the Carba-NP assay
    Chong, Patrick M.
    McCorrister, Stuart J.
    Unger, Mark S.
    Boyd, David A.
    Mulvey, Michael R.
    Westmacott, Garrett R.
    JOURNAL OF MICROBIOLOGICAL METHODS, 2015, 111 : 21 - 23
  • [23] Comparison of the Clinical Outcomes of Patients With Positive Xpert Carba-R Tests for Carbapenemase-Producing Enterobacterales According to Culture Positivity
    Seo, Hyeonji
    Lee, Jeong-Young
    Ryu, Seung Hee
    Kwak, Sun Hee
    Kim, Eun Ok
    Bae, Seongman
    Kim, Min Jae
    Chong, Yong Pil
    Kim, Sung-Han
    Lee, Sang-Oh
    Choi, Sang-Ho
    Sung, Heungsup
    Kim, Mi-Na
    Kim, Yang Soo
    Jung, Jiwon
    OPEN FORUM INFECTIOUS DISEASES, 2022, 9 (01):
  • [24] Comparison between detection power of MBT STAR-Carba test and KBM CIM Tris II for carbapenemase-producing bacteria
    Komatsu, Shoko
    Fujinaga, Chinami
    Nakanishi, Noriko
    JOURNAL OF LABORATORY MEDICINE, 2025, 49 (01) : 53 - 58
  • [25] Rapid Detection of Carbapenemase-Producing Multidrug-Resistant (MDR) Pathogens by Modified Carba NP Test in Ventilator-Associated Pneumonia (VAP) in Elderly Patients
    Pasha, S. A. Rahil
    Yadav, Ruby Suresh Kumar
    Ahmed, Md Iqbal
    Chandra, Pratibha
    CUREUS JOURNAL OF MEDICAL SCIENCE, 2023, 15 (08)
  • [26] Performance comparison of BD Phoenix CPO detect panel with Cepheid Xpert Carba-R assay for the detection of carbapenemase-producing Klebsiella pneumoniae isolates
    Tuzemen, Nazmiye Ulku
    Onal, Ugur
    Merdan, Osman
    Akca, Bekir
    Ener, Beyza
    Akalin, Halis
    Ozakin, Cuneyt
    BMC MICROBIOLOGY, 2024, 24 (01):
  • [27] Performance Evaluation of MALDI-TOF MS MBT STAR-BL Versus In-House Carba NP Testing for the Rapid Detection of Carbapenemase Activity in Escherichia coli and Klebsiella pneumoniae Strains
    Akyar, Isin
    Ayas, Meltem Kaya
    Karatuna, Onur
    MICROBIAL DRUG RESISTANCE, 2019, 25 (07) : 985 - 990
  • [28] Comparison of the performance of three variations of the Carbapenem Inactivation Method (CIM, modi fied CIM [mCIM] and in-house method (iCIM)) for the detection of carbapenemase-producing Enterobacterales and non-fermenters
    Howard, J. C.
    Creighton, J.
    Ikram, R.
    Werno, A. M.
    JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, 2020, 21 : 78 - 82