Biology of Drug-Induced Gingival Hyperplasia: In Vitro Study of the Effect of Nifedipine on Human Fibroblasts

被引:30
|
作者
Lauritano, Dorina [1 ]
Moreo, Giulia [1 ]
Della Vella, Fedora [2 ]
Palmieri, Annalisa [3 ]
Carinci, Francesco [4 ]
Petruzzi, Massimo [2 ]
机构
[1] Univ Milano Bicocca, Dept Med & Surg, Ctr Neurosci Milan, I-20126 Milan, Italy
[2] Univ Bari, Interdisciplinary Dept Med, I-70121 Bari, Italy
[3] Univ Bologna, Dept Expt Diagnost & Specialty Med, Via Belmoro 8, I-40126 Bologna, Italy
[4] Univ Ferrara, Dept Translat Med, I-44121 Ferrara, Italy
来源
APPLIED SCIENCES-BASEL | 2021年 / 11卷 / 07期
关键词
drug-induced gingival overgrowth; nifedipine; human gingival fibroblasts; hypertension therapy side effects;
D O I
10.3390/app11073287
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Background: It has been proven that the antihypertensive agent nifedipine can cause gingival overgrowth as a side effect. The aim of this study was to analyze the effects of pharmacological treatment with nifedipine on human gingival fibroblasts activity, investigating the possible pathogenetic mechanisms that lead to the onset of gingival enlargement. Methods: The expression profile of 57 genes belonging to the "Extracellular Matrix and Adhesion Molecules" pathway, fibroblasts' viability at different drug concentrations, and E-cadherin levels in treated fibroblasts were assessed using real-time Polymerase Chain Reaction, PrestoBlue (TM) cell viability test, and an enzyme-linked immunoassay (ELISA), respectively. Results: Metalloproteinase 24 and 8 (MMP24, MMP8) showed significant upregulation in treated cells with respect to the control group, and cell adhesion gene CDH1 (E-cadherin) levels were recorded as increased in treated fibroblasts using both real-time PCR and ELISA. Downregulation was observed for transmembrane receptors ITGA6 and ITGB4, the basement membrane constituent LAMA1 and LAMB1, and the extracellular matrix protease MMP11, MMP16, and MMP26. Conclusions: The obtained data suggested that the pathogenesis of nifedipine-induced gingival overgrowth is characterized by an excessive accumulation of collagen due to the inhibition of collagen intracellular and extracellular degradation pathways.
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页数:10
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