Photochemically induced dynamic nuclear polarization in photosystem I of plants observed by 13C magic-angle spinning NMR

被引：49

作者：

Alia

Roy, E

Gast, P

van Gorkom, HJ

de Groot, HJM

Jeschke, G

Matysik, J

机构：

[1] Gorlaeus Labs, Leiden Inst Chem, NL-2300 RA Leiden, Netherlands

[2] Huygens Lab, Dept Biophys, NL-2300 RA Leiden, Netherlands

[3] Max Planck Inst Polymer Res, D-55021 Mainz, Germany

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2004年 / 126卷 / 40期

关键词：

D O I：

10.1021/ja048051+

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Photochemically induced dynamic nuclear polarization (photo-CIDNP) has been observed in photosystem I of spinach by C-13 magic angle spinning solid-state NMR under continuous illumination with white light. An almost complete set of chemical shifts of the aromatic ring carbons of a single Chl a molecule has been obtained which is assigned to the P2-cofactor of the primary electron donor P700. Since all light-induced C-13 NMR signals appear to be emissive, a predominance of the three-spin mixing mechanism over the differential decay mechanism is proposed. The origin of the strong contribution of the three-spin mixing mechanism and the differences with photosystem II are discussed.

引用

收藏

页码：12819 / 12826

页数：8

相关论文

共 68 条

[1] Ultrahigh field MAS NMR dipolar correlation spectroscopy of the histidine residues in light-harvesting complex II from photosynthetic bacteria reveals partial internal charge transfer in the B850/His complex [J].

Alia ;

Matysik, J ;

Soede-Huijbregts, C ;

Baldus, M ;

Raap, J ;

Lugtenburg, J ;

Gast, P ;

van Gorkom, HJ ;

Hoff, AJ ;

de Groot, HJM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (20) :4803-4809

[2] COPPER ENZYMES IN ISOLATED CHLOROPLASTS - POLYPHENOLOXIDASE IN BETA-VULGARIS [J].

ARNON, DI .

PLANT PHYSIOLOGY, 1949, 24 (01) :1-15

[3] EFFECTS OF CATIONS UPON CHLOROPLAST MEMBRANE SUBUNIT INTERACTIONS AND EXCITATION-ENERGY DISTRIBUTION [J].

ARNTZEN, CJ ;

DITTO, CL .

BIOCHIMICA ET BIOPHYSICA ACTA, 1976, 449 (02) :259-274

[4] HETERONUCLEAR DECOUPLING IN ROTATING SOLIDS [J].

BENNETT, AE ;

RIENSTRA, CM ;

AUGER, M ;

LAKSHMI, KV ;

GRIFFIN, RG .

JOURNAL OF CHEMICAL PHYSICS, 1995, 103 (16) :6951-6958

[5] Magnetic-field-induced orientation of photosynthetic reaction centers as revealed by time-resolved W-band EPR of spin-correlated radical pairs [J].

Berthold, T ;

Bechtold, M ;

Heinen, U ;

Link, G ;

Poluektov, O ;

Utschig, L ;

Tang, J ;

Thurnauer, MC ;

Kothe, G .

JOURNAL OF PHYSICAL CHEMISTRY B, 1999, 103 (49) :10733-10736

[6] CHEMICALLY-INDUCED DYNAMIC ELECTRON POLARIZATION IN CHLOROPLASTS AT ROOM-TEMPERATURE - EVIDENCE FOR TRIPLET-STATE PARTICIPATION IN PHOTOSYNTHESIS [J].

BLANKENSHIP, R ;

MCGUIRE, A ;

SAUER, K .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (12) :4943-4947

[7]

BOENDER GJ, 1996, THSIS U LEIDEN NETHE

[8] IDENTIFICATION OF MULTIPLE COMPONENTS IN THE INTERMEDIARY ELECTRON CARRIER COMPLEX OF PHOTOSYSTEM-I [J].

BONNERJEA, J ;

EVANS, MCW .

FEBS LETTERS, 1982, 148 (02) :313-316

[9] The g-factor anisotropy of plant chlorophyll a• [J].

Bratt, PJ ;

Poluektov, OG ;

Thurnauer, MC ;

Krzystek, J ;

Brunel, LC ;

Schrier, J ;

Hsiao, YW ;

Zerner, M ;

Angerhofer, A .

JOURNAL OF PHYSICAL CHEMISTRY B, 2000, 104 (30) :6973-6977

[10] FTIR study of the primary electron donor of photosystem I (P700) revealing delocalization of the charge in P700+ and localization of the triplet character in 3P700 [J].

Breton, J ;

Nabedryk, E ;

Leibl, W .

BIOCHEMISTRY, 1999, 38 (36) :11585-11592

← 1 2 3 4 5 6 7 →