Regulation of TGF-β-mediated endothelial-mesenchymal transition by microRNA-27

被引：42

作者：

Suzuki, Hiroshi I. ^{[1
,2
]}

Katsura, Akihiro ^{[1
]}

Mihira, Hajime ^{[1
]}

Horie, Masafumi ^{[3
]}

Saito, Akira ^{[3
]}

Miyazono, Kohei ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Med, Dept Mol Pathol, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1130033, Japan

[2] MIT, David H Koch Inst Integrat Canc Res, 500 Main St,76-417, Cambridge, MA 02139 USA

[3] Univ Tokyo, Grad Sch Med, Dept Resp Med, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1130033, Japan

来源：

JOURNAL OF BIOCHEMISTRY | 2017年 / 161卷 / 05期

基金：

日本学术振兴会;

关键词：

TGF-beta; EndMT; microRNA; alpha-SMA; Elk1; CELL FATE; ANGIOGENESIS; MECHANISMS; EXPRESSION; CLUSTERS;

D O I：

10.1093/jb/mvx017

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Multiple microRNAs (miRNAs) regulate epithelial-mesenchymal transition and endothelial-mesenchymal transition (EndMT). Here we report that microRNA-27b (miR-27b) positively regulates transforming growth factor-beta (TGF-beta)-induced EndMT of MS-1 mouse pancreatic microvascular endothelial cells. TGF-beta induced miR-23b/24-1/27b expression, and inhibition of miR-27 suppressed TGF-beta-mediated induction of mesenchymal genes. Genome-wide miRNA target analysis revealed that miR-27 targets Elk1, which acts as a competitive inhibitor of myocardin-related transcription factor-serum response factor signalling and as a myogenic repressor. miR-27b was also found to regulate several semaphorin receptors including Neuropilin 2, Plexin A2 and Plexin D1. These results suggest important roles of miR-27 in TGF-beta-driven EndMT.

引用

页码：417 / 420

页数：4

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