Protein-responsive rolling circle amplification as a tandem template to drive amplified transduction of fluorescence signal probes for highly sensitive immunoassay

被引：40
作者：
Yang, Wenting [1 ]
Shen, Yu [1 ]
Zhang, Danyang [1 ]
Xu, Wenju [1 ]
机构：
[1] Southwest Univ, Sch Chem & Chem Engn, Minist Educ, Key Lab Luminescent & Real Time Analyt Chem, Chongqing 400715, Peoples R China
来源：
CHEMICAL COMMUNICATIONS | 2018年 / 54卷 / 72期
基金：
中国国家自然科学基金;
关键词：
DNA STRAND DISPLACEMENT; HELIX MOLECULAR SWITCH; PROXIMITY LIGATION; NUCLEIC-ACIDS; ULTRASENSITIVE DETECTION; SENSING PLATFORM; CANCER; BIOSENSOR; NANOMACHINE; TELOMERASE;
D O I：
10.1039/c8cc04395g
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Proximity binding-induced DNA assemblies possessing outstanding flexibility and diversity can provide great accessibility for output translation of amplifiable DNA probes or directly detectable signals. Based on a novel protein-responsive DNA assembly constructed via proximity binding-initiated rolling circle amplification (RCA), we present a sensitive and specific fluorescence resonance energy transfer (FRET) strategy for the immunoassay of mucin 1 (MUC1). The resultant RCA product containing numerous and continuous repeats as the tandem template accelerates the transduction of signal probes through the disassembly of triple-helix stem structure (THSS), achieving a significantly amplified FRET signal readout.
引用
收藏
页码：10195 / 10198
页数：4
相关论文
共 41 条
  • [1] Low Dielectric Polyetherimides Derived from Bis[4-(4-(4-aminophenoxy)- 2-tert- butylphenoxy) phenyl] Sulfone and 4,4'-Bis[4( 4- aminophenoxy)-2-tert-butylphenoxy] perfluorobiphenyl
    Chen, Chien Han
    Lin, Ching Hsuan
    Wang, Meng Wei
    Juang, Tzong Yuan
    [J]. ACS OMEGA, 2017, 2 (08): : 4800 - 4807
  • [2] Aptamer-Based Detection of Epithelial Tumor Marker Mucin 1 with Quantum Dot-Based Fluorescence Readout
    Cheng, Alan K. H.
    Su, Huaipeng
    Wang, Andrew
    Yu, Hua-Zhong
    [J]. ANALYTICAL CHEMISTRY, 2009, 81 (15) : 6130 - 6139
  • [3] A signal on aptamer-based electrochemical sensing platform using a triple-helix molecular switch
    Du, Yudan
    Mao, Yinfei
    He, Xiaoxiao
    Wang, Kemin
    Yan, Genping
    Liu, Jinquan
    Wang, Yonghong
    [J]. ANALYTICAL METHODS, 2014, 6 (16) : 6294 - 6300
  • [4] Protein detection using proximity-dependent DNA ligation assays
    Fredriksson, S
    Gullberg, M
    Jarvius, J
    Olsson, C
    Pietras, K
    Gústafsdóttir, SM
    Östman, A
    Landegren, U
    [J]. NATURE BIOTECHNOLOGY, 2002, 20 (05) : 473 - 477
  • [5] Green N M, 1975, Adv Protein Chem, V29, P85, DOI 10.1016/S0065-3233(08)60411-8
  • [6] Cytokine detection by antibody-based proximity ligation
    Gullberg, M
    Gústafsdóttir, SM
    Schallmeiner, E
    Jarvius, J
    Bjarnegård, M
    Betsholtz, C
    Landegren, U
    Fredriksson, S
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (22) : 8420 - 8424
  • [7] Rolling Circle Amplification-Templated DNA Nanotubes Show Increased Stability and Cell Penetration Ability
    Hamblin, Graham D.
    Carneiro, Karina M. M.
    Fakhoury, Johans F.
    Bujold, Katherine E.
    Sleiman, Hanadi F.
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2012, 134 (06) : 2888 - 2891
  • [8] A graphene oxide-based fluorescent aptasensor for the turn-on detection of epithelial tumor marker mucin 1
    He, Yue
    Lin, Yi
    Tang, Hongwu
    Pang, Daiwen
    [J]. NANOSCALE, 2012, 4 (06) : 2054 - 2059
  • [9] Integration of isothermal amplification with quantum dot- based fluorescence resonance energy transfer for simultaneous detection of multiple microRNAs
    Hu, Juan
    Liu, Ming-hao
    Zhang, Chun-yang
    [J]. CHEMICAL SCIENCE, 2018, 9 (18) : 4258 - 4267
  • [10] Novel electrochemical aptamer biosensor based on an enzyme-gold nanoparticle dual label for the ultrasensitive detection of epithelial tumour marker MUC1
    Hu, Rong
    Wen, Wei
    Wang, Qingling
    Xiong, Huayu
    Zhang, Xiuhua
    Gu, Haoshuang
    Wang, Shengfu
    [J]. BIOSENSORS & BIOELECTRONICS, 2014, 53 : 384 - 389
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