Molecular characterization of a novel fusarivirus infecting the plant-pathogenic fungus Alternaria solani

被引：10

作者：

Gong, WeiJie ^{[1
]}

Liu, Hong ^{[1
]}

Zhu, XiaoFei ^{[1
]}

Zhao, ShiYun ^{[1
]}

Cheng, JiaLe ^{[1
]}

Zhu, Hongjian ^{[1
]}

Zhong, Jie ^{[1
]}

Zhou, Qian ^{[1
]}

机构：

[1] Hunan Agr Univ, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Nongda Rd 1, Changsha 410128, Hunan, Peoples R China

来源：

ARCHIVES OF VIROLOGY | 2021年 / 166卷 / 07期

关键词：

STRANDED-RNA MYCOVIRUS; HYPOVIRULENCE; SEQUENCE;

D O I：

10.1007/s00705-021-05105-y

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A novel mycovirus belonging to the proposed family "Fusariviridae" was discovered in Alternaria solani by sequencing a cDNA corresponding to double-stranded RNA extracted from this phytopathogenic fungus. The virus was tentatively named "Alternaria solani fusarivirus 1" (AsFV1). AsFV1 has a single-stranded positive-sense (+ssRNA) genome of 6845 nucleotides containing three open reading frames (ORFs) and a poly(A) tail. The largest ORF, ORF1, encodes a large polypeptide of 1,556 amino acids (aa) with conserved RNA-dependent RNA polymerase and helicase domains. The ORF2 and ORF3 have overlapping regions, encoding a putative protein of 522 amino acids (aa) and a putative protein of 105 amino acids (aa), respectively, both of unknown function. A multiple sequence alignment and phylogenetic analysis revealed that AsFV1 could be a new member of the "Fusariviridae". This is the first report of the full-length nucleotide sequence of a fusarivirus that infects Alternaria solani.

引用

页码：2063 / 2067

页数：5