Molecular characterization of a novel fusarivirus infecting the plant-pathogenic fungus Alternaria solani

被引:10
作者
Gong, WeiJie [1 ]
Liu, Hong [1 ]
Zhu, XiaoFei [1 ]
Zhao, ShiYun [1 ]
Cheng, JiaLe [1 ]
Zhu, Hongjian [1 ]
Zhong, Jie [1 ]
Zhou, Qian [1 ]
机构
[1] Hunan Agr Univ, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Nongda Rd 1, Changsha 410128, Hunan, Peoples R China
关键词
STRANDED-RNA MYCOVIRUS; HYPOVIRULENCE; SEQUENCE;
D O I
10.1007/s00705-021-05105-y
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A novel mycovirus belonging to the proposed family "Fusariviridae" was discovered in Alternaria solani by sequencing a cDNA corresponding to double-stranded RNA extracted from this phytopathogenic fungus. The virus was tentatively named "Alternaria solani fusarivirus 1" (AsFV1). AsFV1 has a single-stranded positive-sense (+ssRNA) genome of 6845 nucleotides containing three open reading frames (ORFs) and a poly(A) tail. The largest ORF, ORF1, encodes a large polypeptide of 1,556 amino acids (aa) with conserved RNA-dependent RNA polymerase and helicase domains. The ORF2 and ORF3 have overlapping regions, encoding a putative protein of 522 amino acids (aa) and a putative protein of 105 amino acids (aa), respectively, both of unknown function. A multiple sequence alignment and phylogenetic analysis revealed that AsFV1 could be a new member of the "Fusariviridae". This is the first report of the full-length nucleotide sequence of a fusarivirus that infects Alternaria solani.
引用
收藏
页码:2063 / 2067
页数:5
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