A visual chip-based coimmunoprecipitation technique for analysis of protein-protein interactions

被引：2
|
作者：
Chen, Qing [2 ]
Liu, Qiongming [2 ]
Li, Zhoumin [3 ]
Zhong, Wenying [3 ]
He, Wei [2 ]
Xu, Danke [1 ,2 ]
机构：
[1] Nanjing Univ, Sch Chem & Chem Engn, Key Lab Analyt Chem Life Sci MOE, Nanjing 210093, Peoples R China
[2] Beijing Inst Radiat Med, Beijing Proteome Res Ctr, State Key Lab Prote, Beijing 102206, Peoples R China
[3] China Pharmaceut Univ, Dept Analyt Chem, Nanjing 210009, Peoples R China
来源：
ANALYTICAL BIOCHEMISTRY | 2010年 / 404卷 / 02期
关键词：
Protein-protein interactions; Silver enhancement detection; Protein microarrays; Coimmunoprecipitation; MICROARRAYS; SCALE;
D O I：
10.1016/j.ab.2010.05.003
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Here we report a visual chip-based coimmunoprecipitation (vChip-coIP) platform for analysis of protein-protein interactions (PPIs) by combining advantages of an antibody microarray, traditional coIP, and a silver enhancement detection method. The chip was fabricated by spotting anti-Flag antibody on aldehyde-modified slides, and the resulting platform could assay immunoprecipitate from a small amount of crude cell lysates containing Flag-bait and Myc-prey. The interaction signals are visible using biotinylated anti-Myc antibody and colloidal gold-labeled streptavidin followed by a silver enhancement detection method. It is shown that vChip-coIP is a simple, cost-effective, and highly efficient platform for the comprehensive study of PPIs. (C) 2010 Elsevier Inc. All rights reserved.
引用
收藏
页码：244 / 246
页数：3
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