Micropropagation of guava (Psidium guajava']java L.)

A foolproof method of micropropagation through in vitro shoot bud culture has been developed in guava cv. Allahabad Safeda. The stock plant of guava was maintained at shade net house (75% shade) and pruned severely to encourage new growth. Around 3 cm long shoots were excised and defoliated. The explants were pretreated in solution containing 0.1% Carbendazime and 100 mg/L PVP for I hour and then washed with Tween-20 wetting agent. The pretreated explants were further treated with 11902 0-1% for 5 minutes aseptically followed by six washing in autoclaved distilled water. The sterilized explants were cultured on MS medium supplemented with BAP (3.0 mg/L) for shoot bud induction and proliferation. The proliferated shoots were then subcultured on MS medium containing 10 mg/L IBA for rhizogenesis. The rooted plants were finally shifted to autoclaved coconut husk fortified with 1/2 MS salt mixture for acclimatization. Acclimatized plantlets of guava cv. Allahabad Safeda were planted.