CXCR4 positive bone mesenchymal stem cells migrate to human endothelial cell stimulated by ox-LDL via SDF-1α/CXCR4 signaling axis

被引：33
作者：
Li, Mincai [1 ,2 ]
Yu, Jun [1 ]
Li, Yan [1 ]
Li, Dujuan [1 ]
Yan, Dan [1 ]
Qu, Zhiling [1 ]
Ruan, Qiurong [1 ]
机构：
[1] Huazhong Univ Sci & Technol, Inst Pathol, Tongji Hosp, Tongji Med Coll, Wuhan 430030, Peoples R China
[2] Xianning Coll, Dept Pathol, Sch Med, Xianning 437100, Peoples R China
来源：
EXPERIMENTAL AND MOLECULAR PATHOLOGY | 2010年 / 88卷 / 02期
关键词：
CXCR4; Stem cell; SDF-1; alpha; Migration; Adhesion; PROGENITOR CELLS; IN-VITRO; NEOINTIMAL HYPERPLASIA; VASCULAR INJURY; CHEMOKINE SDF-1; TUMOR-CELLS; MARROW; RECRUITMENT; EXPRESSION; LIGAND;
D O I：
10.1016/j.yexmp.2009.12.001
中图分类号：
R36 [病理学];
学科分类号：
100104 ;
摘要：
Background: Bone mesenchymal stem cells (BMSCs) are attractive candidates for cell based therapies to cardiovascular disease such as infarction and atherosclerosis; however, the mechanisms responsible for stem cell chemotaxis and homing remain unknown. Chemokine stromal cell-derived factor 1 (SDF-1 alpha) is involved in the process of atherogenesis. This study was aimed at investigating whether the SDF-1 alpha of human umbilical vein endothelial cells (HUVECs) plays a role in migration of BM-derived CXCR4(+) (receptor for SDF-1 alpha) stem cells. Methods: HUVECs were cultured from human umbilical cords and was treated with ox-LDL The mRNA and protein expression of SDF-1 alpha was detected in HUVECs. CXCR4(+)BMSCs from bone marrow were isolated and were tested by migration and adhesion assays. Results: It was found that ox-LDL induced HUVECs to increase the mRNA and protein expression of SDF-1 alpha. Ox-LDL increased the migratory and adhesion response of CXCR4(+)BMSCs. When the neutralizing SDF-1 alpha antibody abrogated the secreted SDF-1 alpha, the migration and adhesion response of CXCR4(+)BMSCs markedly decreased. Conclusions: Our data indicated that the endothelial cells (ECs) stimulated by ox-LDL could increase the BMSCs migratory response via SDF-1 alpha/CXCR4 signaling axis. These findings provide a new paradigm for biological effects of ox-LDL and have implications for novel stem cell therapeutic strategies for atherosclerosis. (C) 2009 Elsevier Inc. All rights reserved.
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页码：250 / 255
页数：6
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