Design of a liquid crystal-based aptasensing platform for ultrasensitive detection of tetracycline

We develop a novel label-free liquid crystal (LC) aptasensor based on intrinsic properties of nematic LCs for ultra-sensitive detection of tetracycline. The aptasensor is assembled by immobilizing aptamers onto the glass slide modified with both homeotropic alignment and silane coupling agents. Designed aptasensor makes use of the target-induced aptamer conformational switching and disruption of the orientation of LCs which lead to an obvious change of the optical appearance from a dark to a bright response. We describe the optimized condition for maintaining the homeotropic orientation of LCs, which are suitable for the tetracycline detection. The average gray-scale intensities of polarizing optical microscopy images were calculated to quantitatively detect tetracycline concentrations. The aptasensor works especially at trace level of tetracycline as low as 0.5 pM. Moreover, the LC aptasensor was successfully used to detect tetracycline in the real milk sample. According to the results, the proposed LC aptasensor for tetracycline detection is simple, ultra-sensitive, label free and ease of preparation.