Expression of smooth-muscle actin in cultured cells from human plasmacytoid myoepithelioma

被引:15
作者
Jaeger, RG [1 ]
de Oliveira, PT [1 ]
Jaeger, MMM [1 ]
de Araujo, VC [1 ]
机构
[1] Univ Sao Paulo, Sch Dent, Dept Oral Pathol, Sao Paulo, Brazil
来源
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTICS | 1997年 / 84卷 / 06期
关键词
D O I
10.1016/S1079-2104(97)90369-3
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Objective. The definition of plasmacytoid myoepithelioma, a neoplasm exhibiting myoepithelial differentiation, has been recently questioned. To better understand the histogenesis of this neoplasm, we searched for myoepithelial markers in histologic sections of plasmacytoid myoepithelioma and in a cell line (M1) derived from this same neoplasm. Study design. Expression of vimentin, pan-keratin (AE-3) and smooth-muscle actin was studied by immunohistochemistry in paraffin-embedded tissue and by immunofluorescence in M1 cells. Results. Plasmacytoid myoepithelioma tumor sections showed vimentin and AE-3 reactivity, but evidence of smooth-muscle actin was not seen. The cell line derived from this tumor also produced vimentin and cytokeratin. In addition, all cultured cells expressed smooth-muscle actin. Conclusion. We demonstrated that cells derived from a case of plasmacytoid myoepithelioma appear to show full myoepithelial differentiation in vitro. Thus, they are myoepithelial-like cells in nature. The lack of myogenous differentiation in vivo could be due to an inhibitory process mediated by the extracellular matrix.
引用
收藏
页码:663 / 667
页数:5
相关论文
共 36 条
[1]  
ARAUJO VC, 1990, EUR ARCH OTO-RHINO-L, V247, P252
[2]  
ARAUJO VC, 1996, EUR J CANC B, V32, P14
[3]  
Araujo Vera Cavalcanti De, 1994, Oral Surgery Oral Medicine Oral Pathology, V77, P387
[4]   MYOEPITHELIOMAS OF THE HEAD AND NECK - CASE-REPORT AND REVIEW [J].
BARNES, L ;
APPEL, BN ;
PEREZ, H ;
ELATTAR, AM .
JOURNAL OF SURGICAL ONCOLOGY, 1985, 28 (01) :21-28
[5]   PATHOLOGY CONSULTATION - MYOEPITHELIOMA [J].
BATSAKIS, JG .
ANNALS OF OTOLOGY RHINOLOGY AND LARYNGOLOGY, 1985, 94 (05) :523-524
[6]  
CHENG J, 1992, CANCER, V69, P2631, DOI 10.1002/1097-0142(19920601)69:11<2631::AID-CNCR2820691103>3.0.CO
[7]  
2-P
[8]  
COONS AH, 1950, J EXP MED, V91, P1, DOI 10.1084/jem.91.1.1
[9]   PLEOMORPHIC ADENOMA .1. ULTRASTRUCTURAL ORGANIZATION OF EPITHELIAL REGIONS [J].
DARDICK, I ;
VANNOSTRAND, AWP ;
JEANS, MTD ;
RIPPSTEIN, P ;
EDWARDS, V .
HUMAN PATHOLOGY, 1983, 14 (09) :780-797
[10]   PLEOMORPHIC ADENOMA .2. ULTRASTRUCTURAL ORGANIZATION OF STROMAL REGIONS [J].
DARDICK, I ;
VANNOSTRAND, AWP ;
JEANS, MTD ;
RIPPSTEIN, P ;
EDWARDS, V .
HUMAN PATHOLOGY, 1983, 14 (09) :798-809