Identification of a Tc1-like transposon integration site in the genome of the flounder (Platichthys flesus): A novel use of an inverse PCR method

被引：2

作者：

Pocwierz-Kotus, Anita ^{[1
]}

Burzynski, Artur ^{[1
]}

Wenne, Roman ^{[1
]}

机构：

[1] Polish Acad Sci, Inst Oceanol, Dept Genet & Marine Biotechnol, PL-81712 Sopot, Poland

来源：

MARINE GENOMICS | 2010年 / 3卷 / 01期

关键词：

Tc1-like transposons; HMM; LINES; Non-LTR retrotransposons; TCL-LIKE TRANSPOSONS; FAMILY; FISH; ELEMENTS; VERTEBRATE; SEQUENCES; SOFTWARE; DATABASE; GENES;

D O I：

10.1016/j.margen.2010.03.001

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The inverse PCR method has been developed and applied employed for the identification of the integration sites of the Tc1-like transposons in the genome of the flounder, Platichthys flesus. One Id-like insertion instance was recognized and characterized, demonstrating an efficiency of the method for determining of transposon integration sites. The similarity of the sequence flanking transposon (SFT) to reverse transcriptase sequences (RVT) was demonstrated. It is likely that the insertion took place within currently degenerated LINE (long interspersed nuclear elements) retrotransposon. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：45 / 50

页数：6

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