Droplet Combinations: A Scalable Microfluidic Platform for Biochemical Assays

被引：4

作者：

Cliffe, Finola E. ^{[1
]}

Lyons, Mark ^{[1
]}

Murphy, Daniel C. ^{[1
]}

McInerney, Lisa ^{[1
]}

Hurley, Niall ^{[1
]}

Galvin, Michael A. ^{[1
,2
]}

Mulqueen, Jane ^{[1
,2
]}

Bible, Lorraine B. ^{[1
,2
]}

Marella, Claudio ^{[1
]}

Kelleher, Michael ^{[2
]}

O'Sullivan, Anne ^{[2
]}

Fearnhead, Howard O. ^{[3
]}

O'Connell, Enda ^{[3
]}

Davies, Mark ^{[2
]}

机构：

[1] Hooke Bio Ltd, Shannon, Co Clare, Ireland

[2] Univ Limerick, Bernal Inst, Limerick, Ireland

[3] NUI Galway, Biomed Sci, Pharmacol & Therapeut, Galway, Ireland

来源：

SLAS TECHNOLOGY | 2020年 / 25卷 / 02期

关键词：

high-throughput screening; microfluidics; drug combinations; droplets; OIL/WATER INTERFACE; NANOLITER PLUGS; ADSORPTION; REAGENTS; LIFE; BSA;

D O I：

10.1177/2472630319883830

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Droplet-based microfluidics holds enormous potential for transforming high-throughput drug screening. Miniaturization through droplets in combination with automation contributes to reduce reagent use and analysis time as well as minimizing or eliminating labor-intensive steps leading to associated reductions in cost. In this paper, we demonstrate the potential of automated and cost-effective microfluidic droplet-generating technology in the context of an enzymatic activity assay for screening collagenase inhibitors. Experimental results show reproducible and accurate creation and mixing of droplet combinations resulting in biochemical data comparable to data produced by an industry standard instrument. This microfluidic platform that can generate and combine multiple droplets represents a promising tool for high-throughput drug screening.

引用

页码：140 / 150

页数：11

共 47 条

[1] Ultrahigh-throughput screening in drop-based microfluidics for directed evolution [J].