Extraction and hydrolysis parameters for determination of quercetin in Hypericum perforatum

被引：18

作者：

Biesaga, M. ^{[1
]}

Stafiej, A. ^{[1
]}

Pyrzynska, K. ^{[1
]}

机构：

[1] Warsaw Univ, Dept Chem, PL-02093 Warsaw, Poland

来源：

CHROMATOGRAPHIA | 2007年 / 65卷 / 11-12期

关键词：

column liquid chromatography; extraction and hydrolysis parameters; flavonoids; Hypericum perforatum;

D O I：

10.1365/s10337-007-0204-z

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The optimum conditions for extraction of rutin and quercetin from Hypericum perforatum were investigated. The best efficiency of extraction was achieved with aqueous methanol 40-80% (v/v). For quercetin analysis as aglycone the effect of acid concentration and hydrolysis time on the extraction recovery were also studied. Hydrolysis for 5 min in the presence of 2.8 mol L-1 HCl as well as for 10 min with 1.1 mol L-1 HCl efficiently released quercetin from rutin. The content of quercetin-3-O-glycosides (rutin, hyperoside and quercetrin) and quercetin aglycone as well as chlorogenic and caffeic acids in H. perforatum leaves and flowers were determined by HPLC with photodiode-array detection and confirmed by electrospray mass spectrometry.

引用

页码：701 / 706

页数：6

共 29 条

[1] Determination of major constituents in St. John's Wort under different extraction conditions [J].