The origin of amniotic fluid monocytes/macrophages in women with intra-amniotic inflammation or infection

被引：36

作者：

Gomez-Lopez, Nardhy ^{[1
,2
,3
,4
]}

Romero, Roberto ^{[2
,3
,5
,6
,7
,8
,9
,10
]}

Leng, Yaozhu ^{[1
,2
,3
]}

Xu, Yi ^{[1
,2
,3
]}

Slutsky, Rebecca ^{[2
,3
]}

Levenson, Dustyn ^{[1
,2
,3
]}

Pacora, Percy ^{[1
,2
,3
]}

Jung, Eunjung ^{[1
,2
,3
]}

Panaitescu, Bogdan ^{[1
]}

Hsu, Chaur-Dong ^{[1
,2
,3
,11
]}

机构：

[1] Wayne State Univ, Dept Obstet & Gynecol, Sch Med, 275 E Hancock, Detroit, MI 48201 USA

[2] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Perinatol Res Branch, Div Obstet & Maternal Fetal Med, Div Intramural Res,US Dept Hlth & Human Serv,NIH, Bethesda, MD 20892 USA

[3] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Perinatol Res Branch, Div Obstet & Maternal Fetal Med, Div Intramural Res,US Dept Hlth & Human Serv,NIH, Detroit, MI 48202 USA

[4] Wayne State Univ, Sch Med, Dept Immunol Microbiol & Biochem, Detroit, MI 48202 USA

[5] NICHD, Perinatol Res Branch, DHHS, Hutzel Womens Hosp,NIH, 3990 John R,Box 4, Detroit, MI 48201 USA

[6] Univ Michigan, Dept Obstet & Gynecol, Ann Arbor, MI 48109 USA

[7] Michigan State Univ, Dept Epidemiol & Biostat, E Lansing, MI 48824 USA

[8] Wayne State Univ, Ctr Mol Med & Genet, Detroit, MI 48202 USA

[9] Detroit Med Ctr, Detroit, MI 48201 USA

[10] Florida Int Univ, Dept Obstet & Gynecol, Miami, FL 33199 USA

[11] Wayne State Univ, Sch Med, Dept Physiol, Detroit, MI 48201 USA

来源：

JOURNAL OF PERINATAL MEDICINE | 2019年 / 47卷 / 08期

基金：

美国国家卫生研究院;

关键词：

acute chorioamnionitis; clinical chorioamnionitis; fetal inflammatory response; funisitis; innate immune cells; interleukin-6; labor; microbial invasion of the amniotic cavity; pregnancy; preterm labor; term labor; PRETERM PREMATURE RUPTURE; BLOOD-CELL COUNT; POLYMERASE-CHAIN-REACTION; GAMMA-INDUCING FACTOR; C-REACTIVE PROTEIN; MICROBIAL INVASION; UREAPLASMA-UREALYTICUM; CLINICAL-SIGNIFICANCE; GRAM STAIN; HISTOLOGIC CHORIOAMNIONITIS;

D O I：

10.1515/jpm-2019-0262

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Background: Monocytes, after neutrophils, are the most abundant white blood cells found in the amniotic cavity of women with intra-amniotic inflammation/infection. However, the origin of such cells has not been fully investigated. Herein, we determined (1) the origin of amniotic fluid monocytes/macrophages from women with intra-amniotic inflammation/infection, (2) the relationship between the origin of amniotic fluid monocytes/macrophages and preterm or term delivery and (3) the localization of monocytes/macrophages in the placental tissues. Methods: Amniotic fluid samples (n =16) were collected from women with suspected intra-amniotic inflammation or infection. Amniotic fluid monocytes/macrophages were purified by fluorescence-activated cell sorting, and DNA fingerprinting was performed. Blinded placental histopathological evaluations were conducted. Immunohistochemistry was performed to detect CD14+ monocytes/macrophages in the placental tissues. Results: DNA fingerprinting revealed that (1) 56.25% (9/16) of amniotic fluid samples had mostly fetal monocytes/macrophages, (2) 37.5% (6/16) had predominantly maternal monocytes/macrophages and (3) one sample (6.25% [1/16]) had a mixture of fetal and maternal monocytes/macrophages. (4) Most samples with predominantly fetal monocytes/macrophages were from women who delivered early preterm neonates (77.8% [7/9]), whereas all samples with mostly maternal monocytes/macrophages or a mixture of both were from women who delivered term or late preterm neonates (100% [7/7]). (5) Most of the women included in this study presented acute maternal and fetal inflammatory responses in the placenta (85.7% [12/14]). (6) Women who had mostly fetal monocytes/macrophages in amniotic fluid had abundant CD14+ cells in the umbilical cord and chorionic plate, whereas women with mostly maternal amniotic fluid monocytes/macrophages had abundant CD14+ cells in the chorioamniotic membranes. Conclusion: Amniotic fluid monocytes/macrophages can be of either fetal or maternal origin, or a mixture of both, in women with intra-amniotic inflammation or infection. These immune cells could be derived from the fetal and maternal vasculature of the placenta.

引用

页码：822 / 840

页数：19

共 167 条

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