ELECTROFORMATION OF NEUTRAL AND NEGATIVELY CHARGED PHOSPHOLIPID GIANT VESICLES UNDER PHYSIOLOGICAL CONDITIONS

被引：0

作者：

Pavliv, Janez Ivan ^{[1
,3
]}

Genova, Julia ^{[2
]}

Zheliaskova, Antonia ^{[2
]}

Iglic, Ales ^{[1
]}

Mitov, Marin D. ^{[2
]}

机构：

[1] Univ Ljubljana, Biophys Lab, Fac Elect Engn, Ljubljana 1000, Slovenia

[2] Bulgarian Acad Sci, Lab Liquid Crystals, Inst Solid State Phys, BU-1784 Sofia, Bulgaria

[3] Univ Ljubljana, Fac Hlth Sci, Ljubljana 1000, Slovenia

来源：

COMPTES RENDUS DE L ACADEMIE BULGARE DES SCIENCES | 2010年 / 63卷 / 04期

关键词：

electroformation; charged phospholipidis; giant vesicles; BILAYER VESICLES; LIPID-MEMBRANES; FLUCTUATIONS; ELASTICITY; SONICATION; LIPOSOMES;

D O I：

暂无

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We propose a new simple and easy electroformation method for preparation of neutral and negatively charged giant unilamellar vesicles in different aqueous solutions that are of great importance in medicine, biology and electrochemistry. We manage to produce repetitively a subsequent amount of vesicles without, any visible defects in pure phosphate buffer solution, pure physiological solution and in mixed sugar-buffer solution. The bending elastic modulus for one of the studied systems is preliminarily calculated on the base of 4 different vesicles, formed by the proposed electroformation procedure. For negatively charged liposomes cardiolipin is used in two different mass ratios. In this short communication we briefly describe our preparation procedure and the biological importance of our achievement.

引用

页码：497 / 502

页数：8

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