ELECTROFORMATION OF NEUTRAL AND NEGATIVELY CHARGED PHOSPHOLIPID GIANT VESICLES UNDER PHYSIOLOGICAL CONDITIONS

We propose a new simple and easy electroformation method for preparation of neutral and negatively charged giant unilamellar vesicles in different aqueous solutions that are of great importance in medicine, biology and electrochemistry. We manage to produce repetitively a subsequent amount of vesicles without, any visible defects in pure phosphate buffer solution, pure physiological solution and in mixed sugar-buffer solution. The bending elastic modulus for one of the studied systems is preliminarily calculated on the base of 4 different vesicles, formed by the proposed electroformation procedure. For negatively charged liposomes cardiolipin is used in two different mass ratios. In this short communication we briefly describe our preparation procedure and the biological importance of our achievement.