Probe with large Stokes shift for effective cysteine imaging in living cells

被引:17
|
作者
Zou, Fengxia [1 ]
Wang, Chun [2 ,3 ]
Song, Wenwu [1 ]
Shen, Lujie [1 ]
Xu, Runsheng [1 ]
Wang, Miao [1 ,3 ]
Wang, Minmin [1 ,3 ]
Sun, Tongming [1 ]
Wang, Jin [1 ,3 ]
机构
[1] Nantong Univ, Sch Chem & Chem Engn, Nantong 226019, Peoples R China
[2] Nantong Univ, Sch Text & Clothing, Nantong 226001, Peoples R China
[3] Nantong Key Lab Intelligent & New Energy Mat, Nantong 226001, Peoples R China
基金
中国国家自然科学基金;
关键词
Cys detection; Coumarin; Large Stokes shift; ESIPT; Cell imaging; ENABLES SIMULTANEOUS DETECTION; ESIPT FLUORESCENT-PROBE; TURN-ON DETECTION; SELECTIVE DETECTION; DISCRIMINATION; CYS;
D O I
10.1016/j.saa.2021.119775
中图分类号
O433 [光谱学];
学科分类号
0703 ; 070302 ;
摘要
A new fluorescence probe L, which featured with a large Stokes shift (216 nm), was designed for sensitive detection of cysteine (Cys) and a potential sensing mechanism derived from excited state intramolecular proton transfer (ESIPT) was proposed. More importantly, probe L exhibits higher selective to Cys than other amino acid due to its specific cyclization between acrylate group and Cys. Meanwhile, the probe L shows a low detection limit of 8.82 x 10(8) M, which is enough for detecting Cys in organisms. Furthermore, this probe displays high biocompatibility and can image Cys in living cells. (C) 2021 Elsevier B.V. All rights reserved.
引用
收藏
页数:6
相关论文
共 50 条
  • [41] An isophorone-fused near-infrared fluorescent probe with a large Stokes shift for imaging endogenous nitroxyl in living cells and zebrafish
    Wei, Chao
    Wang, Xiaofen
    Li, Xueyan
    Jia, Xu
    Hao, Xinya
    Zhang, Jiangyan
    Zhang, Pingzhu
    Li, Xiaoliu
    SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2020, 227
  • [42] A novel fluorescent probe with large Stokes shift for two-photon imaging of biothiols in living cells, liver tissues and tumor tissues
    Dai, Xi
    Kong, Xiuqi
    Lin, Weiying
    DYES AND PIGMENTS, 2017, 142 : 306 - 314
  • [43] A facile fluorescent probe with a large Stokes shift for sequentially detecting copper and sulfide in 100% aqueous solution and imaging them in living cells
    Wang, Huan
    Shi, Dong-Lei
    Li, Jin
    Tang, Hao-Yang
    Li, Jian
    Guo, Yuan
    SENSORS AND ACTUATORS B-CHEMICAL, 2018, 256 : 600 - 608
  • [44] A novel near-infrared probe with large Stokes shift for the detection of viscosity changes in living cells
    Zhang, Liang
    Guo, Rui
    Hu, Qian
    Lin, Weiying
    JOURNAL OF LUMINESCENCE, 2021, 233
  • [45] Phenothiazine-based fluorescence probe for ratiometric imaging of hydrazine in living cells with remarkable Stokes shift
    Qiu, Xiao-Yang
    Liu, Shu-Juan
    Hao, Yuan-Qiang
    Sun, Jing-Wen
    Chen, Song
    SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2020, 227
  • [46] A red probe with large Stokes shift for imaging the viscosity of lipid droplets
    Li, Zhenru
    Zhang, Qikun
    Huang, Hongjin
    Shu, Yang
    Wang, Jianhua
    DYES AND PIGMENTS, 2024, 229
  • [47] A ratiometric fluorescent probe with large Stokes shift and emission shift for sensing hydrazine in living organisms
    Su, Hang
    Wang, Jingpei
    Yue, Xiuxiu
    Wang, Benhua
    Song, Xiangzhi
    Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 2022, 274
  • [48] A ratiometric fluorescent probe with large Stokes shift and emission shift for sensing hydrazine in living organisms
    Su, Hang
    Wang, Jingpei
    Yue, Xiuxiu
    Wang, Benhua
    Song, Xiangzhi
    SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2022, 274
  • [49] A near-infrared fluorescent probe for imaging endogenous carbon monoxide in living systems with a large Stokes shift
    Gong, Shengyi
    Hong, Jiaxin
    Zhou, Enbo
    Feng, Guoqiang
    TALANTA, 2019, 201 : 40 - 45
  • [50] Development of a novel near-infrared fluorescence light-up probe with a large Stokes shift for sensing of cysteine in aqueous solution, living cells and zebrafish
    Zhang, Xiaoyu
    Liu, Heng
    Ma, Yingying
    Qu, Wangbo
    He, Hanping
    Zhang, Xiuhua
    Wang, Shengfu
    Sun, Qi
    Yu, Fabiao
    DYES AND PIGMENTS, 2019, 171