Multimodal assessment of SERS nanoparticle biodistribution post ingestion reveals new potential for clinical translation of Raman imaging

被引:30
作者
Campbell, Jos L. [1 ,2 ,3 ]
SoRelle, Elliott D. [1 ,4 ,5 ]
Ilovich, Ohad [1 ,3 ,6 ]
Liba, Orly [1 ,7 ]
James, Michelle L. [1 ,3 ]
Qiu, Zhen [1 ,3 ,8 ]
Perez, Valerie [1 ,3 ,9 ]
Chan, Carmel T. [1 ,3 ]
de la Zerda, Adam [1 ,4 ,5 ,7 ]
Zavaleta, Cristina [1 ,3 ]
机构
[1] Stanford Univ, Mol Imaging Program, 318 Campus Dr, Stanford, CA 94305 USA
[2] RMIT Univ, 124 Latrobe St, Melbourne, Vic 3000, Australia
[3] Stanford Univ, Dept Radiol, 1201 Welch Rd, Stanford, CA 94305 USA
[4] Stanford Univ, Biophys Program, 291 Campus Dr, Stanford, CA 94305 USA
[5] Stanford Univ, Dept Struct Biol, 299 Campus Dr, Stanford, CA 94305 USA
[6] InviCRO LLC, Imaging Serv & Software, 27 Drydock Ave, Boston, MA 02210 USA
[7] Stanford Univ, Dept Elect Engn, 350 Serra Mall, Stanford, CA 94305 USA
[8] Dept Pediat, 300 Pasteur Dr H310, Stanford, CA 94305 USA
[9] Stanford Univ, Dept Chem Engn, 443 Via Ortega, Stanford, CA 94305 USA
基金
美国国家科学基金会;
关键词
SERS nanoparticles; Biodistribution; Raman imaging; Systemic toxicity; MicroPET; SILICA NANOPARTICLES; SPECTRAL ENDOSCOPY; CANCER; SPECTROSCOPY; SCATTERING; TOXICITY; RATS;
D O I
10.1016/j.biomaterials.2017.04.045
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Despite extensive research and development, new nano-based diagnostic contrast agents have faced major barriers in gaining regulatory approval due to their potential systemic toxicity and prolonged retention in vital organs. Here we use five independent biodistribution techniques to demonstrate that oral ingestion of one such agent, gold-silica Raman nanoparticles, results in complete clearance with no systemic toxicity in living mice. The oral delivery mimics topical administration to the oral cavity and gastrointestinal (GI) tract as an alternative to intravenous injection. Biodistribution and clearance profiles of orally (OR) vs. intravenously (IV) administered Raman nanoparticles were assayed over the course of 48 h. Mice given either an IV or oral dose of Raman nanoparticles radiolabeled with approximately 100 mu Ci (3.7MBq) of Cu-64 were imaged with dynamic microPET immediately post nanoparticle administration. Static microPET images were also acquired at 2 h, 5 h, 24 h and 48 h. Mice were sacrificed post imaging and various analyses were performed on the excised organs to determine nanoparticle localization. The results from microPET imaging, gamma counting, Raman imaging, ICP-MS, and hyperspectral imaging of tissue sections all correlated to reveal no evidence of systemic distribution of Raman nanoparticles after oral administration and complete clearance from the GI tract within 24 h. Paired with the unique signals and multiplexing potential of Raman nanoparticles, this approach holds great promise for realizing targeted imaging of tumors and dysplastic tissues within the oral cavity and GI-tract. Moreover, these results suggest a viable path for the first translation of high-sensitivity Raman contrast imaging into clinical practice. (C) 2017 Elsevier Ltd. All rights reserved.
引用
收藏
页码:42 / 52
页数:11
相关论文
共 48 条
  • [21] NIH, 2016, SURV EP END RES SEER
  • [22] Quantitative analysis using Raman spectrometry
    Pelletier, MJ
    [J]. APPLIED SPECTROSCOPY, 2003, 57 (01) : 20A - 42A
  • [23] Ravi SB, J CLIN DIAGN RES, V8
  • [24] MicroPET-based biodistribution of quantum dots in living mice
    Schipper, Meike L.
    Cheng, Zhen
    Lee, Sheen-Woo
    Bentolila, Laurent A.
    Iyer, Gopal
    Rao, Jianghong
    Chen, Xiaoyuan
    Wu, Anna M.
    Weiss, Shimon
    Gambhir, Sanjiv S.
    [J]. JOURNAL OF NUCLEAR MEDICINE, 2007, 48 (09) : 1511 - 1518
  • [25] Silica Nanoparticles as Substrates for Chelator-free Labeling of Oxophilic Radioisotopes
    Shaffer, Travis M.
    Wall, Matthew A.
    Harmsen, Stefan
    Longo, Valerie A.
    Drain, Charles Michael
    Kircher, Moritz F.
    Grimm, Jan
    [J]. NANO LETTERS, 2015, 15 (02) : 864 - 868
  • [26] Shen Z., 2016, MOL PHARM
  • [27] A hyperspectral method to assay the microphysiological fates of nanomaterials in histological samples
    SoRelle, Elliott D.
    Liba, Orly
    Campbell, Jos L.
    Dalal, Roopa
    Zavaleta, Cristina L.
    de la Zerda, Adam
    [J]. ELIFE, 2016, 5
  • [28] Gold Nanoparticles: A Revival in Precious Metal Administration to Patients
    Thakor, A. S.
    Jokerst, J.
    Zavaleta, C.
    Massoud, T. F.
    Gambhir, S. S.
    [J]. NANO LETTERS, 2011, 11 (10) : 4029 - 4036
  • [29] Thakor A. S., 2016, J NUCL MED OFFICIAL
  • [30] Nanooncology: The Future of Cancer Diagnosis and Therapy
    Thakor, Avnesh S.
    Gambhir, Sanjiv S.
    [J]. CA-A CANCER JOURNAL FOR CLINICIANS, 2013, 63 (06) : 395 - 418