Comparison of mango genomic DNA isolation methods for next generation sequencing

被引：0

作者：

Sharma, Nimisha ^{[1
]}

Singh, A. K. ^{[1
]}

Srivastav, Manish ^{[1
]}

Singh, B. P. ^{[1
]}

Mahto, A. K. ^{[1
]}

Singh, N. K. ^{[1
]}

机构：

[1] Indian Agr Res Inst, Div Fruits & Hort Technol, New Delhi 110012, India

来源：

INDIAN JOURNAL OF HORTICULTURE | 2014年 / 71卷 / 02期

关键词：

DNA extraction; ddRAD sequencing; Mangifera indica L;

D O I：

暂无

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

Six different methods of DNA isolation namely, CTAB, CTAB with PVP, Qiagene DNA extraction Mini and Maxi kits, CTAB with addition of PVP and purification using PCI followed by Qiagen Genomic-tip 500/G were compared for recovery of quality DNA from mango leaves. The higher yield (1375.0 ng/ mu l) of good quality (A(260/280) and A(260/230) 1.80 & 1.90, respectively) DNA was obtained with modified CTAB method with addition of PVP (MW, 40,000) followed by purification using phenol: chloroform: isoamylalcohol 25:24:1 and Qiagen Genomic-tip 500/G as compared to standard CTAB method (1096.50 ng/ mu l; A(260/280) and A(260/230) 1.40 and & 1.10, respectively). The DNA obtained using modified CTAB method was found suitable for PCR, PacBio, ddRAD sequencing and long-term storage.

引用

页码：260 / 263

页数：4

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