Fabrication and fluorescent labeling of guar gum nanoparticles in a surfactant free aqueous environment

被引:21
作者
Ghosh, Sumanta Kumar [1 ]
Abdullah, Farooque [1 ]
Mukherjee, Arup [1 ]
机构
[1] Univ Calcutta, Dept Chem Technol, Div Pharmaceut & Fine Chem Technol, Kolkata 700009, W Bengal, India
来源
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2015年 / 46卷
关键词
Guar gum nanopartide; Mineral acid; Fluorescence; Cellular imaging; CELLULOSE NANOCRYSTALS;
D O I
10.1016/j.msec.2014.10.080
中图分类号
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号
0805 ; 080501 ; 080502 ;
摘要
Guar gum (GG) is a galactomannan obtained from the seeds of Cyamopsis tetragonoloba. GG polysaccharide is used in a range of technology arenas and its properties in water environment elicited a lot of interest. We report a simple acid hydrolysis technique to derive water dispersible spherical nanoparticles from GG for the first time without any stabilizing surfactant. The particles demonstrated colloidal stability and were observed to carry negative surface charges in aqueous environment The TEM study of guar gum nanoparticles (GGn) indicated a size range of 30-80 nm with a mean at 48.8 nm. Mineral acid at apposite concentration have apparently solubilized the amorphous regions of the polymer chain leaving the crystalline parts. GGn were further covalently functionalized in aqueous environment with fluorescein isothiocyanate (FITC) using a hydroxy-propyl amine spacer group. Nanoparticle fluorescence intensity can be modulated with pH changes and a significant augmentation in the intensity was noticed at the physiologically relevant range of 6-8. New fluorescendy labeled nanoparticles have potential for applications in bioimaging, biomolecular interaction studies and as a pH sensing probe in sub cellular environment. (C) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码:521 / 529
页数:9
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